Human Kallikrein 14 (KLK14) RTU ELISA Kit | KLK14 rtu elisa kit

Human Kallikrein 14 (KLK14) ELISA Kit

Cat. Num. AAA4500608

• Gene Names: KLK14; KLK-L6
• Reactivity: Human
• Synonyms: Kallikrein 14 (KLK14); Human Kallikrein 14 (KLK14) ELISA Kit; KLK-L6; Kallikrein-like protein 6; KLK14 rtu elisa kit

• Reactivity: Human
• Sequence Length: 267

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.044ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLK14 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KLK14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to KLK14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KLK14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of KLK14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 908212436
• NCBI GeneID: 43847
• NCBI Accession #: NP_001298111.1
• NCBI GenBank Nucleotide #: NM_001311182.1
• UniProt Accession #: Q9P0G3; Q1RMZ2; Q6B089; A7UNK5
• Molecular Weight: 29,122 Da
• NCBI Official Full Name: kallikrein-14 preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 14
• NCBI Official Symbol: KLK14
• NCBI Official Synonym Symbols: KLK-L6
• NCBI Protein Information: kallikrein-14
• UniProt Protein Name: Kallikrein-14
• Protein Family: Kallikrein
• UniProt Gene Name: KLK14
• UniProt Synonym Gene Names: KLKL6; hK14; KLK-L6

NCBI Description

This gene encodes a member of the kallikrein subfamily of serine proteases that have diverse physiological functions such as regulation of blood pressure and desquamation. The altered expression of this gene is implicated in the progression of different cancers including breast and prostate tumors. The encoded protein is a precursor that is proteolytically processed to generate the functional enzyme. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis.