Mouse Inosine Triphosphatase (ITPA) RTU ELISA Kit | ITPA rtu elisa kit

Mouse Inosine Triphosphatase (ITPA) ELISA Kit

Cat. Num. AAA4500542

• Gene Names: Itpa; Itp; ITPase; NTPase; AU020102; 2010016I08Rik
• Reactivity: Mouse
• Synonyms: Inosine Triphosphatase (ITPA); Mouse Inosine Triphosphatase (ITPA) ELISA Kit; ITPase; Nucleoside Triphosphate Pyrophosphatase; Inosine Triphosphate Pyrophosphatase; Non-canonical purine NTP pyrophosphatase; Nucleoside-triphosphate diphosphatase; ITPA rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 198

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.049ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITPA rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ITPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ITPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ITPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ITPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31982664
• NCBI GeneID: 16434
• NCBI Accession #: NP_080198.2
• NCBI GenBank Nucleotide #: NM_025922.3
• UniProt Accession #: Q9D892; Q8R0Q8
• Molecular Weight: 21,897 Da
• NCBI Official Full Name: inosine triphosphate pyrophosphatase isoform 1
• NCBI Official Synonym Full Names: inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
• NCBI Official Symbol: Itpa
• NCBI Official Synonym Symbols: Itp; ITPase; NTPase; AU020102; 2010016I08Rik
• NCBI Protein Information: inosine triphosphate pyrophosphatase
• UniProt Protein Name: Inosine triphosphate pyrophosphatase
• UniProt Gene Name: Itpa
• UniProt Synonym Gene Names: ITPase; Inosine triphosphatase; NTPase

Uniprot Description

Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.