Mouse Beta-2 Microglobulin Monoclonal Antibody | anti-B2M antibody

Beta-2 Microglobulin (Renal Failure & Tumor Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439750

• Gene Names: B2M; IMD43
• Reactivity: Human.; Does not react with Non-Human Primates
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: Beta-2 Microglobulin; Monoclonal Antibody; Beta-2 Microglobulin (Renal Failure & Tumor Marker) Mouse Monoclonal Antibody; B2M; Beta 2 microglobin; Beta 2 microglobulin; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; Hdcma22p; anti-B2M antibody

• Host: Mouse
• Reactivity: Human.; Does not react with Non-Human Primates
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: SPM374
• Specificity: Recognizes a protein of 12kDa, identified as beta-2-microglobulin. Beta-2-microglobulin non-covalently associates with the 44kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This antibody reacts with all cell types excluding erythrocytes. The detection of b2-microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 119

• Applicable Applications for anti-B2M antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)
• Cellular Localization: Cell Surface and Extracellular (Secreted)
• Immunogen: Human PBL s from a T-cell acute lymphoblastic leukemia (T-ALL) patient
• Hu-Chromosome Location: 15q21-q22.2
• Positive Control: HL-60 or HeLa cells. Melanomas and Lymphoma. Carcinoma of Stomach, Cervix, Endometrial, Kidney or Colon.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Sparrow RL. Human cell surface antigens defined by monoclonal antibodies. PhD thesis, University of Melbourne, 1983. Betts RL, McKenzie IFC: Monoclonal antibodies to the major histocompatibility antigens. Monoclonal hybridoma antibodies: Techniques and applications. Edited by D. Hurrel. Uniscience series program. C.R.C. Press, Cleveland, OH: 1983, pp. 193-222 Brodsky FM, Parham P. Barnstable CJ, Crumpton MJ, Bodmer WF: Monoclonal antibodies for analysis of the HLA system. lmmunol Rev 47:3, (1979). Leah J. Cosgrove et al.: HLA (Class I) antigens on platelets are involved in platelet function. Immunol. Cell Biol., 66 (1) 69-77 (1988)

NCBI and Uniprot Product Information

• NCBI GI #: 4757826
• NCBI GeneID: 567
• NCBI Accession #: NP_004039.1
• NCBI GenBank Nucleotide #: NM_004048.2
• UniProt Accession #: P61769; P01884; Q540F8; Q6IAT8; Q9UCK0; Q9UD48; Q9UDF4
• Molecular Weight: 12kDa
• NCBI Official Full Name: beta-2-microglobulin
• NCBI Official Synonym Full Names: beta-2-microglobulin
• NCBI Official Symbol: B2M
• NCBI Official Synonym Symbols: IMD43
• NCBI Protein Information: beta-2-microglobulin
• UniProt Protein Name: Beta-2-microglobulin
• Protein Family: Beta-2-microglobulin
• UniProt Gene Name: B2M
• UniProt Entry Name: B2MG_HUMAN

NCBI Description

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Uniprot Description

B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: cytoplasm; early endosome membrane; endoplasmic reticulum lumen; extracellular region; extracellular space; focal adhesion; Golgi apparatus; Golgi membrane; membrane; phagocytic vesicle membrane; plasma membrane

Molecular Function: glycoprotein binding; identical protein binding; protein binding

Biological Process: antibacterial humoral response; antigen processing and presentation of endogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; innate immune response; iron ion homeostasis; positive regulation of protein binding; positive regulation of receptor-mediated endocytosis; positive regulation of T cell cytokine production; regulation of defense response to virus by virus; regulation of immune response; retinal homeostasis

Disease: Hypoproteinemia, Hypercatabolic