Rat anti-Human, Mouse Laminin, gamma 1 (LAMC1) Monoclonal Antibody | anti-LAMC1 antibody

Laminin, gamma 1 (LAMC1) Rat Monoclonal Antibody

Cat. Num. AAA439133

• Gene Names: LAMC1; LAMB2
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: Laminin; gamma 1 (LAMC1); Monoclonal Antibody; gamma 1 (LAMC1) Rat Monoclonal Antibody; LAMB2; LAMC1; Laminin B2 chain; Laminin gamma 1; Laminin gamma 1 chain; anti-LAMC1 antibody

• Host: Rat
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, kappa
• Clone Number: A5
• Specificity: Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta , and gamma chains. This MAb reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This MAb stains uniformly all human and murine basement membranes.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
• Sequence Length: 1609

• Applicable Applications for anti-LAMC1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Basement Membrane
• Immunogen: Murine EHS laminin preparation
• Hu-Chromosome Location: 1q31
• Positive Control: Normal colon or carcinomas
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Laminin Monoclonal Antibody (A5).)

References

Ljubimov AV et. al., Int J Cancer, 1992; 50:562-566. Ljubimov AV et. al. Lab Investigation, 1995; 72:461-473. Folkvord et. al., J Histochem Cytochem, 1989; 37:105-113

NCBI and Uniprot Product Information

• NCBI GI #: 145309326
• NCBI GeneID: 3915
• NCBI Accession #: NP_002284.3
• NCBI GenBank Nucleotide #: NM_002293.3
• UniProt Accession #: P11047; Q5VYE7
• Molecular Weight: 210kDa
• NCBI Official Full Name: laminin subunit gamma-1
• NCBI Official Synonym Full Names: laminin subunit gamma 1
• NCBI Official Symbol: LAMC1
• NCBI Official Synonym Symbols: LAMB2
• NCBI Protein Information: laminin subunit gamma-1
• UniProt Protein Name: Laminin subunit gamma-1
• Protein Family: Laminin
• UniProt Gene Name: LAMC1
• UniProt Synonym Gene Names: LAMB2; S-LAM gamma
• UniProt Entry Name: LAMC1_HUMAN

NCBI Description

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that laminin, gamma 1 chain is necessary for laminin heterotrimer assembly. It has been inferred by analogy with the strikingly similar 3' UTR sequence in mouse laminin gamma 1 cDNA, that multiple polyadenylation sites are utilized in human to generate the 2 different sized mRNAs (5.5 and 7.5 kb) seen on Northern analysis. [provided by RefSeq, Aug 2011]

Uniprot Description

LAMC1: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: basement membrane; extracellular matrix; extracellular region; laminin-1 complex; laminin-10 complex

Molecular Function: extracellular matrix structural constituent

Biological Process: cell adhesion; cell migration; endoderm development; extracellular matrix disassembly; extracellular matrix organization and biogenesis; hemidesmosome assembly; positive regulation of epithelial cell proliferation; protein complex assembly

Disease: Pelvic Organ Prolapse, Susceptibility To