Mouse TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody | anti-TNF antibody

TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody

Cat. Num. AAA439097

• Gene Names: TNF; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
• Reactivity: Human.; Does not react with Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Neutralization Assay
• Synonyms: TNF-alpha (Tumor Necrosis Factor alpha); Monoclonal Antibody; TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody; APC1; Cachectin; Differentiation inducing factor (DIF); Macrophage cytotoxic factor (MCF); Necrosin; TNF alpha; TNF Macrophage Derived; TNF Monocyte Derived; TNF Superfamily Member 2; TNFA; TNFSF2; Tumor necrosis factor ligand superfamily member 2; Tumor Necrosis Factor Precursor; anti-TNF antibody

• Host: Mouse
• Reactivity: Human.; Does not react with Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: J2D10
• Specificity: This antibody neutralises HurTNFamediated cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects mice against toxicity of HurTNFa. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 233

• Applicable Applications for anti-TNF antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Inhibits Tumor Growth, Neutralizes rTNF-alpha Mediated Cytotoxicity, Protects Mice Against Toxicity of rhTNF-alpha
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml); Inhibits Tumor Growth (Order Ab without BSA/Azide); Neutralizes rTNF-alpha Mediated Cytotoxicity (Order Ab without BSA/Azide); Protects Mice Against Toxicity of rhTNF-alpha (Order Ab without BSA/Azide)
• Cellular Localization: Cytoplasmic and extracellular (Secreted)
• Immunogen: Recombinant human TNF-alpha
• Hu-Chromosome Location: 6p21.33
• Positive Control: HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

McLaughlin PJ; Elwood NJ; Russell SM; Andrew SM; McKenzie IF. Properties of monoclonal antibodies to human tumor necrosis factor alpha (TNF alpha). Anticancer Research, 1992, 12(4):1243-6. McLaughlin PJ; Elwood NJ; Ramadi LT, Pica MR, McKenzie IF. Improvement in sensitivity of enzyme-linked immunosorbent assay for tumor necrosis factor. Immunol Cell Biol, 1990, 68:51-5

NCBI and Uniprot Product Information

• NCBI GI #: 25952111
• NCBI GeneID: 7124
• NCBI Accession #: NP_000585.2
• NCBI GenBank Nucleotide #: NM_000594.3
• UniProt Accession #: P01375; O43647; Q9P1Q2; Q9UIV3
• Molecular Weight: 17kDa
• NCBI Official Full Name: tumor necrosis factor
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: TNF
• NCBI Official Synonym Symbols: DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
• NCBI Protein Information: tumor necrosis factor
• UniProt Protein Name: Tumor necrosis factor
• Protein Family: Tumor necrosis factor
• UniProt Gene Name: TNF
• UniProt Synonym Gene Names: TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_HUMAN

NCBI Description

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Uniprot Description

TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Apoptosis; Membrane protein, integral; Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cell surface; external side of plasma membrane; extracellular region; extracellular space; integral to plasma membrane; lipid raft; phagocytic cup; plasma membrane; recycling endosome

Molecular Function: cytokine activity; identical protein binding; protease binding; protein binding; tumor necrosis factor receptor binding

Biological Process: activation of MAPK activity; activation of MAPKKK activity; activation of NF-kappaB transcription factor; caspase activation; chronic inflammatory response to antigenic stimulus; cortical actin cytoskeleton organization and biogenesis; embryonic gut development; I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; inflammatory response; leukocyte tethering or rolling; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; negative regulation of cytokine secretion during immune response; negative regulation of interleukin-6 production; negative regulation of lipid catabolic process; negative regulation of protein complex disassembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of cell adhesion; positive regulation of chemokine biosynthetic process; positive regulation of chemokine production; positive regulation of cytokine production; positive regulation of cytokine secretion; positive regulation of fever; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of JNK activity; positive regulation of MAP kinase activity; positive regulation of membrane protein ectodomain proteolysis; positive regulation of NF-kappaB import into nucleus; positive regulation of NFAT protein import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phagocytosis; positive regulation of programmed cell death; positive regulation of protein amino acid phosphorylation; positive regulation of protein complex assembly; positive regulation of protein complex disassembly; positive regulation of protein kinase activity; positive regulation of protein transport; positive regulation of smooth muscle cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein import into nucleus, translocation; protein kinase B signaling cascade; receptor biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; response to glucocorticoid stimulus; response to salt stress; response to virus; sequestering of triacylglycerol; tumor necrosis factor-mediated signaling pathway

Disease: Asthma, Susceptibility To; Malaria, Susceptibility To; Migraine With Or Without Aura, Susceptibility To, 1