Mouse anti-Human. Erythrocyte Specific Monoclonal Antibody
Erythrocyte Specific
Reactivity
Human.
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Synonyms
Erythrocyte Specific; Monoclonal Antibody; RBC; Red blood cells; Erythroid Marker; Erythrocyte specific protein; anti-Erythrocyte Specific antibody
Host
Mouse
Reactivity
Human.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
SFL23.6
Specificity
Mouse Anti-Human Erythrocytes Clone SFL23.6 MAb is directed against an erythroid cell surface antigen. It shows a well defined reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. Although the exact identity of the antigen has yet to be determined, it has been shown to be distinct from Glycophorin A. This MAb is useful in the erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-Erythrocyte Specific antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistology (IH)
Application Notes
Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunocytochemistry (Frozen) (0.5-1.0ug/ml for 30 minutes at RT) Immunohistology (Frozen) (0.5-1.0ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.
Localization
Cytoplasmic
Positive Control
RBC. Placenta
Immunogen
Hepatocytes from 20-22 week-old human fetus
Quantity and Buffer
0.5 ml at 200ug/ml with BSA and azide
Preparation and Storage
Store at 2 to 8 degree C. Antibody is stable for 24 months.
References
1. Das Gupta, A., Samoszuk, MKI, Papayannopoulou, T, and Stamatoyannopoulos, G: SFL 23.6: A monoclonal antibody reactive with CFU-E, erythroblasts, and erythrocytes. Blood 66:522-526, 1985.
Similar Products
Product Notes
The Erythrocyte Specific (Catalog #AAA438271) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Erythrocyte Specific reacts with Human. and may cross-react with other species as described in the data sheet. AAA Biotech's Erythrocyte Specific can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistology (IH). Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunocytochemistry (Frozen) (0.5-1.0ug/ml for 30 minutes at RT) Immunohistology (Frozen) (0.5-1.0ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the Erythrocyte Specific for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Erythrocyte Specific, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
