SDS-Page
(SDS-PAGE Analysis. Purified POLE3 Mouse Monoclonal Antibody (PCRP-POLE3-3D3). Confirmation of Purity and Integrity of Antibody. )
Mouse anti-Human POLE3/CHRAC17 Monoclonal Antibody | anti-POLE3 antibody
POLE3/CHRAC17 Mouse Monoclonal Antibody [Clone PCRP-POLE3-3D3]
Gene Names
POLE3; p17; YBL1; CHRAC17; CHARAC17
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Synonyms
POLE3/CHRAC17; Monoclonal Antibody; POLE3/CHRAC17 Mouse Monoclonal Antibody [Clone PCRP-POLE3-3D3]; Arsenic transactivated protein; ASTP; CHRAC17; Chromatin accessibility complex 17kDa protein; DNA polymerase II subunit 3; Histone fold protein CHRAC17; HuCHRAC17; Polymerase (DNA directed) epsilon 3 (POLE3); YBL1; anti-POLE3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse/IgG1
Clone Number
PCRP-POLE3-3D3
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-POLE3 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
IF: 1-2ug/ml
Immunogen
Recombinant full-length human POLE3 protein
Cellular Localization
Nucleus
Positive Control
HeLa or K562 cells.
Hu-chromosome Location
9q32
Unigene
108112
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-Page
(SDS-PAGE Analysis. Purified POLE3 Mouse Monoclonal Antibody (PCRP-POLE3-3D3). Confirmation of Purity and Integrity of Antibody. )
SDS-Page
(SDS-PAGE Analysis. Purified POLE3 Mouse Monoclonal Antibody (PCRP-POLE3-3D3). Confirmation of Purity and Integrity of Antibody. )
Related Product Information for anti-POLE3 antibody
DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,860 Da
NCBI Official Full Name
DNA polymerase epsilon subunit 3
NCBI Official Synonym Full Names
polymerase (DNA directed), epsilon 3, accessory subunit
NCBI Official Symbol
POLE3
NCBI Official Synonym Symbols
p17; YBL1; CHRAC17; CHARAC17
NCBI Protein Information
DNA polymerase epsilon subunit 3; CHRAC-17; DNA polymerase II subunit 3; DNA polymerase epsilon p17 subunit; DNA polymerase epsilon subunit p17; arsenic transactivated protein; arsenic-transactivated protein; asTP; chromatin accessibility complex 17 kDa p
UniProt Protein Name
DNA polymerase epsilon subunit 3
Protein Family
UniProt Gene Name
POLE3
UniProt Synonym Gene Names
CHRAC17; AsTP; CHRAC-17; HuCHRAC17
UniProt Entry Name
DPOE3_HUMAN
NCBI Description
POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]
Uniprot Description
POLE3: Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
Protein type: EC 2.7.7.7; DNA replication; Nucleotide Metabolism - purine; Transferase; Nucleotide Metabolism - pyrimidine
Chromosomal Location of Human Ortholog: 9q33
Cellular Component: epsilon DNA polymerase complex; nucleus
Molecular Function: protein binding; protein heterodimerization activity; sequence-specific DNA binding; DNA-directed DNA polymerase activity
Biological Process: DNA replication
Similar Products
Product Notes
The POLE3 pole3 (Catalog #AAA4382137) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The POLE3/CHRAC17 Mouse Monoclonal Antibody [Clone PCRP-POLE3-3D3] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POLE3/CHRAC17 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). FC/FACS: 1-2ug/million cells IF: 1-2ug/ml. Researchers should empirically determine the suitability of the POLE3 pole3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLE3/CHRAC17, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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