Mouse anti-Human 26S proteasome non-ATPase4 Monoclonal Antibody | anti-PSMD4 antibody

26S proteasome non-ATPase regulatory subunit 4

Cat. Num. AAA4381606

• Gene Names: PSMD4; AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: 26S proteasome non-ATPase4; Monoclonal Antibody; 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; AF; AF-1; anti-PSMD4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: CPTC-PSMD4-3
• Specificity: In eukaryotic cells, selective breakdown of cellular proteins is ensured by two distinct pathways. First, appropriate proteins are tagged for degradation by ubiquitination. Second, these multiubiquitinated proteins are degraded by the highly selective 26S Proteasome protein-destroying machinery. At specific stages of development, embryo- and tissue-specific components of the 26S Proteasome are formed, which are termed Rpn10a through Rpn10e, or alternatively pUb-R2 through pUb-R5. All members of this family can be generated by a single Rpn10 gene by developmentally regulated alternative splicing. The pUb-R2 subunit, originally identified as S5a (also designated antisecretory factor and multiubiquitin chain binding protein) is ubiquitously expressed and may perform proteolysis constitutively in a wide variety of cells. pUb-R4 and pUb-R5 may have embryo- or tissue-specific expression and may play specialized roles in early embryonic development.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 377

• Applicable Applications for anti-PSMD4 antibody: Western Blot (WB)
• Application Notes: WB: 1-2ug/ml
• Immunogen: Recombinant full-length Human PSMD4 protein
• Cellular Localization: Nucleus and Cytoplasm
• Hu-chromosome Location: 1q21.3
• Positive Control: Jurkat or HepG2 cells. Liver tissue lysate. Human liver, Tonsil or kidney.
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified PSMD4Mouse Monoclonal Antibody (CPTC-PSMD4-3). Confirmation of Integrity and Purity of Antibody. )

Western Blot (WB)

(Western Blot Analysis of Liver tissue lysate using PSMD4Mouse Monoclonal Antibody (CPTC-PSMD4-3).)

Testing Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using 26S proteasome non-ATPase regulatory subunit 4 Mouse Monoclonal Antibody (CPTC-PSMD4-3). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

References

Lonnroth, I, et al. 1986. Biochim.Biophys. Acta 883: 138-144.

NCBI and Uniprot Product Information

• NCBI GI #: 1709796
• NCBI GeneID: 5710
• UniProt Accession #: P55036
• Molecular Weight: 50kDa
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 4
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 4
• NCBI Official Symbol: PSMD4
• NCBI Official Synonym Symbols: AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 4
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 4
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD4
• UniProt Synonym Gene Names: MCB1; AF; ASF
• UniProt Entry Name: PSMD4_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMD4: Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion. The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively. Directly interacts with NUB1. Interacts with SQSTM1. Interacts with UBQLN4. Belongs to the proteasome subunit S5A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: proteasome complex; nucleoplasm; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle