Mouse anti-Human CD14 Antibody | anti-CD14 antibody

CD14 (Monocyte/Macrophage Marker)

Cat. Num. AAA4381404

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD14; CD14 (Monocyte/Macrophage Marker); Lipopolysaccharide receptor; Mo2; Monocyte Differentiation Antigen 14; Myeloid cell-specific leucine-rich glycoprotein; anti-CD14 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1, kappa
• Clone Number: rLPSR/2408
• Specificity: Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 375

• Applicable Applications for anti-CD14 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: A549, THP-1 cells and human monocytes. Lymph nodes and tonsils.
• Immunogen: Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 5q31.1
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human spleen stained with CD14 Recombinant Mouse Monoclonal Antibody (rLPSR/2408).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human spleen stained with CD14 Recombinant Mouse Monoclonal Antibody (rLPSR/2408).)

SDS-Page

(SDS-PAGE Analysis Purified CD14 Recombinant Mouse Monoclonal Antibody (rLPSR/2408). Confirmation of Purity and Integrity of Antibody.)

References

Simmons, D.L., et al. 1989. Blood 73: 284

NCBI and Uniprot Product Information

• NCBI GI #: 4557417
• NCBI GeneID: 929
• NCBI Accession #: NP_000582.1
• NCBI GenBank Nucleotide #: NP_000582.1
• UniProt Accession #: P08571
• Molecular Weight: 55kDa
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 molecule
• NCBI Official Symbol: CD14
• NCBI Protein Information: monocyte differentiation antigen CD14
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: CD14
• UniProt Entry Name: CD14_HUMAN

NCBI Description

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]