Mouse anti-Human CD2/Lymphocyte Function Antigen 2 (LFA-2) Monoclonal Antibody | anti-CD2/LFA-2 antibody

CD2/Lymphocyte Function Antigen 2 (LFA-2)

Cat. Num. AAA4381380

• Gene Names: CD2; T11; SRBC; LFA-2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab conjugated to CF488
• Synonyms: CD2/Lymphocyte Function Antigen 2 (LFA-2); Monoclonal Antibody; Erythrocyte receptor; Ly-37; Lymphocyte function antigen 2 (LFA-2); LFA-3 receptor; Rosette receptor; SRBC; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5; T11; anti-CD2/LFA-2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: BH1
• Specificity: CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.
• Purity/Purification: Purified Ab conjugated to CF488
• Form/Format: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.
• Sequence Length: 351

• Applicable Applications for anti-CD2/LFA-2 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry: 5ul per test per one million cells (or 5ul per 100ul of whole blood); IF: 1:50-1:100
• Positive Control: 293T or Jurkat cells. Lymph node and tonsil.
• Immunogen: Human CD2 protein
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 1p13.1
• Preparation and Storage: Antibody with azide: store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Wang MY et. al. An effective immuno-magnetic method for bone marrow purging in T cell malignancies. Bone Marrow Transplant. 1992;9(5):319-23.

NCBI and Uniprot Product Information

• NCBI GI #: 156071472
• NCBI GeneID: 914
• NCBI Accession #: NP_001758.2
• NCBI GenBank Nucleotide #: NP_001758.2
• UniProt Accession #: P06729
• Molecular Weight: 50kDa
• NCBI Official Full Name: T-cell surface antigen CD2 isoform 2
• NCBI Official Synonym Full Names: CD2 molecule
• NCBI Official Symbol: CD2
• NCBI Official Synonym Symbols: T11; SRBC; LFA-2
• NCBI Protein Information: T-cell surface antigen CD2
• UniProt Protein Name: T-cell surface antigen CD2
• UniProt Gene Name: CD2
• UniProt Synonym Gene Names: SRBC
• UniProt Entry Name: CD2_HUMAN

NCBI Description

The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene. [provided by RefSeq, Jun 2016]

Uniprot Description

CD2: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.

Protein type: Cell surface; Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: internal side of plasma membrane; cell surface; anchored to plasma membrane; integral to plasma membrane; plasma membrane; extracellular region; intercellular junction; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; receptor activity; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; cell surface receptor linked signal transduction; T cell activation; apoptosis; natural killer cell activation; regulation of T cell differentiation; blood coagulation; lipid raft polarization; positive regulation of tumor necrosis factor production; leukocyte migration; positive regulation of myeloid dendritic cell activation