Rabbit anti-Human, Rat Caldesmon, HMW Polyclonal Antibody | anti-CALD1 antibody

Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker)

Cat. Num. AAA4381353

• Gene Names: CALD1; CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
• Reactivity: Human, Rat
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Caldesmon; HMW; Polyclonal Antibody; HMW (h-Caldesmon) (Smooth Muscle Marker); CAD; CALD1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; CDM; HCAD; LCAD; NAG22; anti-CALD1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120'150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70'80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 538

• Applicable Applications for anti-CALD1 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
• Immunogen: Recombinant human CALD1 protein fragment
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 7q33
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Uterus stained with Caldesmon Rabbit Polyclonal Antibody.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded Rat Uterus stained with Caldesmon Rabbit Polyclonal Antibody.)

References

1. Watanabe, K., Tajino, T., Sekiguchi, M. and Suzuki, T. 2000. H-Caldesmon as a specific marker for smooth muscle tumors. Comparison with other smooth muscle markers in bone tumors. Am. J. Clin. Pathol. 113: 663-668. 2. Frid MG, et al. Phenotypic changes of human smooth muscle cells during development: Late expression of heavy caldesmon and calponin. Dev Biol 1992; 153:18

NCBI and Uniprot Product Information

• NCBI GI #: 4826657
• NCBI GeneID: 800
• NCBI Accession #: NP_004333.1
• NCBI GenBank Nucleotide #: NP_004333.1
• UniProt Accession #: Q05682
• Molecular Weight: 150kDa
• NCBI Official Full Name: caldesmon isoform 2
• NCBI Official Synonym Full Names: caldesmon 1
• NCBI Official Symbol: CALD1
• NCBI Official Synonym Symbols: CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
• NCBI Protein Information: caldesmon
• UniProt Protein Name: Caldesmon
• Protein Family: Caldesmon
• UniProt Gene Name: CALD1
• UniProt Synonym Gene Names: CAD; CDM; CDM
• UniProt Entry Name: CALD1_HUMAN

NCBI Description

This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Caldesmon: a cytoskeletal protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells. May act as a bridge between myosin and actin filaments. Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Five alternatively spliced isoforms have been described.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 7q33

Cellular Component: cytoskeleton; myofibril; plasma membrane; actin cap; cytosol; actin cytoskeleton

Molecular Function: calmodulin binding; myosin binding; tropomyosin binding; actin binding

Biological Process: muscle contraction; cell motility