Mouse anti-Human Calcineurin A/PPP3CA Monoclonal Antibody | anti-PPP3CA antibody

Calcineurin A/PPP3CA

Cat. Num. AAA4381191

• Gene Names: PPP3CA; CALN; CCN1; CNA1; CALNA; IECEE; PPP2B; ACCIID; CALNA1; IECEE1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Calcineurin A/PPP3CA; Monoclonal Antibody; Calcineurin A alpha; Calcineurin A1; Calcineurin A; Calmodulin-dependent calcineurin A subunit alpha isoform; CALN; CALNA1; CAM-PRP catalytic subunit; CCN1; CNA alpha; CNA1; Ppp3ca; Protein phosphatase 2B catalytic subunit (PPP2B); Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform (PPP3CA); anti-PPP3CA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: BF1.1
• Specificity: Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 521

• Applicable Applications for anti-PPP3CA antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: For coating, order antibody without BSA; WB: 1-2ug/ml
• Positive Control: HeLa, MCF-7 or A431 cells. Prostate, Thyroid or Pancreas.
• Immunogen: Recombinant full-length Calcineurin A protein
• Cellular Localization: Cytoplasmic and Cell Surface
• Hu-chromosome Location: 4q24
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859. 2. Zeng H. et al. (2001) Cell 107:617

NCBI and Uniprot Product Information

• NCBI GI #: 1352673
• NCBI GeneID: 5530
• UniProt Accession #: Q08209
• Molecular Weight: 62kDa
• NCBI Official Full Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: protein phosphatase 3 catalytic subunit alpha
• NCBI Official Symbol: PPP3CA
• NCBI Official Synonym Symbols: CALN; CCN1; CNA1; CALNA; IECEE; PPP2B; ACCIID; CALNA1; IECEE1
• NCBI Protein Information: serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• UniProt Protein Name: Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
• Protein Family: Serine/threonine-protein phosphatase
• UniProt Gene Name: PPP3CA
• UniProt Synonym Gene Names: CALNA; CNA
• UniProt Entry Name: PP2BA_HUMAN

Uniprot Description

PPP3CA: a calmodulin-dependent Ser/Thr phosphatase also known calcineurin A alpha. Involved in a wide range of biologic activities, acting as a Ca(2 )-dependent modifier of phosphorylation status. Dephosphorylates HSPB1. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. .

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; Z disc; cytosol; calcineurin complex; sarcolemma

Molecular Function: calmodulin binding; protein dimerization activity; protein binding; enzyme binding; calmodulin-dependent protein phosphatase activity; protein heterodimerization activity; protein serine/threonine phosphatase activity; drug binding; calcium ion binding

Biological Process: regulation of synaptic transmission; positive regulation of NFAT protein import into nucleus; T cell activation; response to amphetamine; skeletal muscle fiber development; protein amino acid dephosphorylation; negative regulation of insulin secretion; calcineurin-NFAT signaling pathway; transition between fast and slow fiber; dephosphorylation; protein import into nucleus; calcium ion transport; innate immune response; positive regulation of transcription from RNA polymerase II promoter; response to calcium ion; regulation of excitatory postsynaptic membrane potential; multicellular organismal response to stress; G1/S transition of mitotic cell cycle