Mouse PLK1 (Marker of Mitosis) Monoclonal Antibody | anti-PLK1 antibody

PLK1 (Marker of Mitosis)

Cat. Num. AAA4381038

• Gene Names: plk1.S; plk; plk1
• Reactivity: Xenopus laevis; Does not react with Human or Mouse.
• Applications: ELISA, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: PLK1 (Marker of Mitosis); Monoclonal Antibody; Cell cycle regulated protein kinase; Polo like kinase 1 (PLK1); Serine/threonine-protein kinase PLK1; STPK13; anti-PLK1 antibody

• Host: Mouse
• Reactivity: Xenopus laevis; Does not react with Human or Mouse.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: AZ44
• Specificity: Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and Cdc5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle; Plk protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Useful for confirming that extracts have maintained CSF status and have not yet been activated into interphase.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 598

• Applicable Applications for anti-PLK1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: For coating, order antibody without BSA; WB: 1-2ug/ml
• Positive Control: Mitotic CSF Xenopus egg extract.
• Immunogen: Recombinant full-length PLK1 Xenopus laevis protein
• Cellular Localization: Nucleus and Cytoplasm.
• Hu-chromosome Location: 9_10S (Xenopus laevis)
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified PLK1 Mouse Monoclonal Antibody (AZ44). Confirmation of Purity and Integrity of Antibody.)

References

Sunkel, C.E. and Glover, D.M. 1988. J. Cell Sci. 89: 25-38.

NCBI and Uniprot Product Information

• NCBI GI #: 148235751
• NCBI GeneID: 380481
• NCBI Accession #: NP_001080788.1
• NCBI GenBank Nucleotide #: NP_001080788.1
• UniProt Accession #: P70032
• Molecular Weight: 66kDa
• NCBI Official Full Name: serine/threonine-protein kinase PLK1
• NCBI Official Synonym Full Names: polo like kinase 1 S homeolog
• NCBI Official Symbol: plk1.S
• NCBI Official Synonym Symbols: plk; plk1
• NCBI Protein Information: serine/threonine-protein kinase PLK1
• UniProt Protein Name: Serine/threonine-protein kinase PLK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: plk1
• UniProt Synonym Gene Names: plx1; PLK-1
• UniProt Entry Name: PLK1_XENLA

Uniprot Description

Function: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates cdc25, pkmyt1/myt1, stag2/sa2, tpx2. Plays multiple essential roles during mitosis. Phosphorylates the N-terminal domain of cdc25, which leads to cyclin b-cdc2 activation and mitotic entry. Also required for organization of bipolar spindles, and for exit from mitosis. Involved in kinetochore functions and sister chromatid cohesion by phosphorylating stag2/sa2. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated by phosphorylation of Thr-201. Ref.4

Subunit structure: Interacts with plk1 and kif2a. Ref.9 Ref.10

Subcellular location: Nucleus. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasm › cytoskeleton › spindle. Midbody

By similarity. Note: Localized at centrosomes at prophase, spindles at metaphase, and at the midbody during cytokinesis. Ref.4

Induction: By growth-stimulating agents

By similarity. Ref.4

Domain: The POLO box domains act as phosphopeptide-binding module that recognize and bind serine-[phosphothreonine/phosphoserine]-(proline/X) motifs. plk1 recognizes and binds docking proteins that are already phosphorylated on these motifs, and then phosphorylates them

By similarity.

Post-translational modification: Activated by phosphorylation on Thr-201 during M phase. Phosphorylated by stk10, leading to activation during oocyte maturation. Ref.3 Ref.4 Ref.5Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Protein levels are down-regulated by proteasomal degradation in anaphase

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 2 POLO box domains.Contains 1 protein kinase domain.