Mouse anti-Human CD64 Monoclonal Antibody | anti-CD64 antibody

CD64

Cat. Num. AAA4380897

• Gene Names: FCGR1A; CD64; FCRI; CD64A; IGFR1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD64; Monoclonal Antibody; CD64A; CD64b; CD64c; Fc fragment of IgG; high affinity Ia; receptor (CD64); FCGR1; FCGR1A; FCGR1B; FCGR1C; FcRI; FcRIB; FCRIC; High affinity immunoglobulin gamma Fc receptor IB; IGFR1; IGFRB; IGFRC; anti-CD64 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 10.1
• Specificity: Three different classes of IgG Fc receptors have been described: Fc stimulation. CD64 plays a putative role in the initiation of cell-mediated cytotoxicity. Thus far, three genes encoding four distinct CD64 transcripts have been described. CD64 has been shown to associate with signal transducing subunit of the high affinity IgE receptor. Src family kinases Hck and Lyn show increased kinase activity and will co-immunoprecipitate with CD64 subsequent to receptor cross linking.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml.
• Sequence Length: 374

• Applicable Applications for anti-CD64 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 1-2ug/ml; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Human tonsil.
• Immunogen: FCG10.1A/CD64 antibody was raised against rheumatoid synovial fluid cells and fibronectin Purified human monocytes.
• Cellular Localization: Cell Membrane
• Hu-chromosome Location: 1q21.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Townsend, S.M., et al. 2003. Infect. Immun. 71: 5871-5880

NCBI and Uniprot Product Information

• NCBI GI #: 50403717
• NCBI GeneID: 2209
• UniProt Accession #: P12314
• Molecular Weight: 43kDa
• NCBI Official Full Name: High affinity immunoglobulin gamma Fc receptor I
• NCBI Official Synonym Full Names: Fc fragment of IgG receptor Ia
• NCBI Official Symbol: FCGR1A
• NCBI Official Synonym Symbols: CD64; FCRI; CD64A; IGFR1
• NCBI Protein Information: high affinity immunoglobulin gamma Fc receptor I
• UniProt Protein Name: High affinity immunoglobulin gamma Fc receptor I
• UniProt Gene Name: FCGR1A
• UniProt Synonym Gene Names: FCG1; FCGR1; IGFR1; IgG Fc receptor I; FcRI; FcgammaRIa
• UniProt Entry Name: FCGR1_HUMAN

NCBI Description

This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

FCGR1A: High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses. Belongs to the immunoglobulin superfamily. FCGR1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21.2-q21.3

Cellular Component: early endosome membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; IgG binding; receptor signaling protein activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response; signal transduction; phagocytosis, engulfment