Flow Cytometry (FC/FACS)
(All lanes : SYIM Mouse mAb at 1/2000 dilutionLane 1 : THP1 cells membrane fractionLane 2 : Jurkat cells membrane fraction as negative controlLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size : 113 KDaObserved band size : 113 KDaBlocking/Dilution buffer : 1x TBST.)
Mouse anti-Human IARS2/SYIM Monoclonal Antibody | anti-IARS2/SYIM antibody
IARS2/SYIM (277) Mouse mAb
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Synonyms
IARS2/SYIM; Monoclonal Antibody; IARS2/SYIM (277) Mouse mAb; IARS2/SYIM(277) Mouse mAb; EC 6.1.1.5; IARS2; IleRS; isoleucine tRNA ligase 2; mitochondrial; Isoleucine--tRNA ligase; isoleucine-tRNA synthetase 2; Isoleucyl-tRNA synthetase; isoleucyl-tRNA synthetase; mitochondrial [Precursor]; mitochondrial isoleucine tRNA synthetase; anti-IARS2/SYIM antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Applicable Applications for anti-IARS2/SYIM antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200
IP: 10 tests
FC/FACS: 1:100-1:200
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human SYIM.
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Flow Cytometry (FC/FACS)
(All lanes : SYIM Mouse mAb at 1/2000 dilutionLane 1 : THP1 cells membrane fractionLane 2 : Jurkat cells membrane fraction as negative controlLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size : 113 KDaObserved band size : 113 KDaBlocking/Dilution buffer : 1x TBST.)
Flow Cytometry (FC/FACS)
(All lanes : SYIM Mouse mAb at 1/2000 dilutionLane 1 : THP1 cells membrane fractionLane 2 : Jurkat cells membrane fraction as negative controlLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size : 113 KDaObserved band size : 113 KDaBlocking/Dilution buffer : 1x TBST.)
Related Product Information for anti-IARS2/SYIM antibody
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.
Product Categories/Family for anti-IARS2/SYIM antibody
Similar Products
Product Notes
The IARS2/SYIM (Catalog #AAA435132) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IARS2/SYIM (277) Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IARS2/SYIM can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IP: 10 tests FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the IARS2/SYIM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IARS2/SYIM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
