Monkey Pulmonary Surfactant Associated Protein A ELISA Kit | SPA elisa kit
Monkey Pulmonary Surfactant Associated Protein A ELISA Kit
Gene Names
SFTPA1; PSP-A; SFTPA
Reactivity
Monkey
Synonyms
Pulmonary Surfactant Associated Protein A; Monkey Pulmonary Surfactant Associated Protein A ELISA Kit; SPA elisa kit
Reactivity
Monkey
Specificity
No significant cross-reactivity or interference between Monkey SPA and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
Assay Type
Sandwich
Detection Range
15.6pg/ml-500pg/ml.
Sensitivity
2.0pg/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated SPA concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey SPA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPA and SPA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for SPA elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SPA concentrations in Monkey serum, plasma and other body fluids. Using Purified Monkey SPA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPA and SPA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,702 Da
NCBI Official Full Name
pulmonary surfactant-associated protein A
NCBI Official Symbol
SFTPA1
NCBI Official Synonym Symbols
PSP-A; SFTPA
NCBI Protein Information
pulmonary surfactant-associated protein A; PSAP; SP-A
UniProt Protein Name
Pulmonary surfactant-associated protein A
Protein Family
UniProt Gene Name
SFTPA1
UniProt Synonym Gene Names
SFTP1; SFTPA; PSAP; PSP-A; SP-A
UniProt Entry Name
SFTPA_PIG
Uniprot Description
Function: In presence of calcium ions, PSAP binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.
Subunit structure: Oligomeric complex of 6 set of homotrimers.
Subcellular location: Secreted › extracellular space › extracellular matrix. Secreted › extracellular space › surface film.
Miscellaneous: Pulmonary surfactant consists of 90% lipid and 10% protein. There are 4 surfactant-associated proteins: 2 collagenous, carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small hydrophobic proteins (SP-B and SP-C).
Sequence similarities: Belongs to the SFTPA family.Contains 1 C-type lectin domain.Contains 1 collagen-like domain.
Research Articles on SPA
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Product Notes
The Monkey SPA sftpa1 (Catalog #AAA043130) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA043130 ELISA Kit recognizes Monkey SPA. It is sometimes possible for the material contained within the vial of "Pulmonary Surfactant Associated Protein A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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