Sphingosine-1-Phosphate Receptor 5 (S1PR5) Cell Line | S1PR5 cell line

Sphingosine-1-Phosphate Receptor 5 (S1PR5) ACTOne Stable Cell Line, HEK293

• Gene Names: S1PR5; EDG8; S1P5; Edg-8; SPPR-1; SPPR-2
• Synonyms: Sphingosine-1-Phosphate Receptor 5 (S1PR5); Sphingosine-1-Phosphate Receptor 5 (S1PR5) ACTOne Stable Cell Line; HEK293; Sphingosine-1-Phosphate Receptor 5 (S1PR5) ACTOneTM Stable Cell Line; S1PR5 cell line

• Host: HEK-293 CNG cells

• Application Notes: cAMP dependent human S1PR5 receptor cell based assay; cell based high-throughput screening of human S1PR5 receptor agonists/antagonists
• Detection: FlexStation or Microplate reader
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130 degree C, preferably in liquid nitrogen vapor, until ready for use.

Testing Data

Testing Data

(Figure 1. Response of ACTOneTM S1PR5 cell line & parental cell line to S1PACTOneTM S1PR5 cells and parental cells were plated overnight in 20 ul culture mediumon a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loading solution(membrane potential dye kit. After 2 hours of incubation at room temperature, two readingswere obtained prior to and 30 min after the addition of S1P. Ratios of the two readings (F/F0) are plotted inthe figure.A. Dose response curve of S1P in ACTOneTM S1PR5 cell line. EC50 = 36 nM in the presence ofPDE inhibitor Ro 20-1724 and ?–adren degree Ceptor agonist isoproterenol.B. Parental cells do not respond to S1P.)

Related Product Information for S1PR5 cell line

Introduction: Introduction: S1PR5 is a G-protein-coupled receptor which binds the bioactive signaling molecule sphingosine 1-phosphate (S1P).S1PR5 belongs to a sphingosine-1-phosphate receptor subfamily comprising five members (S1PR5-5).They areexpressed in a wide variety of tissues, with each subtype exhibiting a different cell specificity, although they are found attheir highest density on leuk degree Cytes. S1PR5 may play a regulatory role in the transformation of radial glial cells intoastr degree Cytes and may affect proliferative activity of these cells.
Description: Human S1PR5 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human S1PR5. HEK-293 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levelsand consequently result in ion flux (often detectable by calcium-responsive dye) and cell membranedepolarization which can be easily measured with fluorescent Membrane Potential Dye. The assayallows both end-point and kinetic measurement of intracellular cAMP changes with a FDSS, FLIPR, or a fluorescencemicroplate reader.
This cell line is a HEK-293-CNG cell line that expresses a recombinant human S1PR5. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization.

Product Categories/Family for S1PR5 cell line

Membrane Receptor Stable Cell Lines; ACTOne GPCR Stable Cell Lines; Sphingosine-1-Phosphate Receptor 5 (S1PR5) ACTOne Stable Cell Line

NCBI and Uniprot Product Information

• NCBI GI #: 261278306
• NCBI GeneID: 53637
• NCBI Accession #: NP_001159687.1
• NCBI GenBank Nucleotide #: NM_001166215.1
• UniProt Accession #: Q9H228; Q6NW11
• Molecular Weight: 32,279 Da
• NCBI Official Full Name: sphingosine 1-phosphate receptor 5
• NCBI Official Synonym Full Names: sphingosine-1-phosphate receptor 5
• NCBI Official Symbol: S1PR5
• NCBI Official Synonym Symbols: EDG8; S1P5; Edg-8; SPPR-1; SPPR-2
• NCBI Protein Information: sphingosine 1-phosphate receptor 5
• UniProt Protein Name: Sphingosine 1-phosphate receptor 5
• Protein Family: Sphingosine 1-phosphate receptor
• UniProt Gene Name: S1PR5
• UniProt Synonym Gene Names: EDG8; S1P receptor 5; S1P5; S1P receptor Edg-8
• UniProt Entry Name: S1PR5_HUMAN

NCBI Description

The lysosphingolipid sphingosine 1-phosphate (S1P) regulates cell proliferation, apoptosis, motility, and neurite retraction. Its actions may be both intracellular as a second messenger and extracellular as a receptor ligand. S1P and the structurally related lysolipid mediator lysophosphatidic acid (LPA) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors (e.g., EDG1; MIM 601974) are S1P receptors; others (e.g., EDG2; MIM 602282) are LPA receptors.[supplied by OMIM, Mar 2008]

Uniprot Description

EDG8: Receptor for the lysosphingolipid sphingosine 1- phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins. May play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: integral to membrane; plasma membrane

Biological Process: regulation of neuron differentiation