Enterokinase (rEK) Recombinant Protein | rEK recombinant protein
Recombinant Enterokinase (rEK)
Synonyms
Enterokinase (rEK); Recombinant Enterokinase (rEK); Recombinant Enterokinase; rEK recombinant protein
Host
Yeast
Unit Definition
One unit of rEK is the amount of enzyme that will cleave 20ug of thioredoxin-chloramphenicci acetyl transferase fusion protein containing an enterokinase cleavage site (Asp-Asp-Asp-Asp-Lys) to 90% completion at 37 degree C in 16 hours
Assay Conditions
Recombinant EK in 50mM Tris-HCl, pH 8.0, 1mM CaCl2, 0.1% Tween-20, 20ug of fusion protein, and 1 unit rEK in a 30ul reaction volume incubated at 37 degree C.
Non-Specific Protease Activity Assay
A non-specific protease activity assay of rEK was performed using azocasein as substrate. The results show that rEK contains less than background levels of non-specific protease.
Storage Buffer
rEK in 50mM PBNa, pH 8.0, 0.5M NaCl and 50% glycerol
Preparation and Storage
Store at -20 degree C
Related Product Information for rEK recombinant protein
Recombinant Enterokinase (rEK) is the catalytic subunit of bovine enterokinase, which is expressed by the yeast Pichia pastoris and purified to yield a high enzyme activity preparation. rEK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence.
Product Categories/Family for rEK recombinant protein
Similar Products
Product Notes
The rEK (Catalog #AAA430250) is a Recombinant Protein produced from Yeast and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Enterokinase (rEK), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
