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Immunohistochemistry (IHC) (Biotinylated (1ug/ml) staining of Human Thyroid Gland lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)

anti-Human thyroid peroxidase Antibody | anti-TPO antibody

Goat anti-thyroid peroxidase, Biotinylated Antibody

Gene Names
TPO; MSA; TPX; TDH2A
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
thyroid peroxidase; Goat anti-thyroid peroxidase; Biotinylated Antibody; Goat Anti-thyroid peroxidase Antibody; TPO; MSA; TDH2A; TPX; thyroid microsomal antigen; thyroperoxidase; anti-TPO antibody
Ordering
For Research Use Only!
Reactivity
Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
933
Applicable Applications for anti-TPO antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:32000.
WB: Approx 110kDa band observed in Human Thyroid Gland lysates. Recommended concentration: 1-3ug/ml.
Immunogen Sequence
Peptide with sequence ELRCGKHQAVGTSPQ., from the C Terminus of the protein sequence according to NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1.
Peptide Sequence + C
ELRCGKHQAVGTSPQ.
Epitope
C Terminus
Note
This antibody is expected to recognize all reported isoforms (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1). Reported variants represent identical protein: NP_000538.3, NP_001193673.1 Reported variants represent identical protein: NP_783650.1, NP_001193674.1
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Biotinylated (1ug/ml) staining of Human Thyroid Gland lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)

Immunohistochemistry (IHC) (Biotinylated (1ug/ml) staining of Human Thyroid Gland lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
103kDa
NCBI Official Full Name
thyroid peroxidase isoform a
NCBI Official Synonym Full Names
thyroid peroxidase
NCBI Official Symbol
TPO
NCBI Official Synonym Symbols
MSA; TPX; TDH2A
NCBI Protein Information
thyroid peroxidase
UniProt Protein Name
Thyroid peroxidase
Protein Family
UniProt Gene Name
TPO
UniProt Synonym Gene Names
TPO

NCBI Description

This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]

Uniprot Description

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.

Research Articles on TPO

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Product Notes

The TPO tpo (Catalog #AAA423755) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-thyroid peroxidase, Biotinylated Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's thyroid peroxidase can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Pep-ELISA: antibody detection limit dilution 1:32000. WB: Approx 110kDa band observed in Human Thyroid Gland lysates. Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the TPO tpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "thyroid peroxidase, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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