Immunofluorescence (IF)
((1ug/ml) staining of PFA-perfused cryosection of Mouse embryo E13.5. Primary incubation overnight at 4C. Alexa Fluor 488 detection (green) in combination with nuclear DAPI staining (blue). Data obtained by Ben Jerry Gonzales from Nissim Ben-Arie's lab, Dept. of Cell and Developmental Biology, The Hebrew University of Jeruzalem, Israel.)
Goat Ferd3l Polyclonal Antibody | anti-Ferd3l antibody
Goat anti-Ferd3l (aa56-68) Antibody
Gene Names
Ferd3l; Nato; fer3; Nato3; Mnato3; Ntwist; N-twist; bHLHa31
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Ferd3l; Polyclonal Antibody; Goat anti-Ferd3l (aa56-68) Antibody; Fer3-like (Drosophila); Mnato3; N-twist; Nato; Nato3; Ntwist; bHLHa31; fer3; basic helix-loop-helix protein N-twist; fer3-like protein; nephew of atonal 3; neuronal twist; Ferd3l antibody; Fer3-like (Drosophila) antibody; Mnato3 antibody; N-twist antibody; Nato antibody; Nato3 antibody; Ntwist antibody; bHLHa31 antibody; fer3 antibody; basic helix-loop-helix protein N-twist antibody; fer3-like protein antibody; nephew of atonal 3 antibody; neuronal twist antibody; Ferd3l (aa56-68); anti-Ferd3l antibody
Host
Goat
Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QFDERYQEVEGDE
Sequence Length
168
Applicable Applications for anti-Ferd3l antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: Frozen sections of Mouse embryo E13.5 shows staining of the floor plate of the developing spinal cord. Recommended concentration, 1-3ug/ml.
Immunohistochemistry: Frozen sections of Mouse embryo E13.5 shows staining of the floor plate of the developing spinal cord. Recommended concentration, 1-3ug/ml.
Immunogen
Peptide with sequence C-QFDERYQEVEGDE, from the internal region of the protein sequence according to NP_277057.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Immunofluorescence (IF)
((1ug/ml) staining of PFA-perfused cryosection of Mouse embryo E13.5. Primary incubation overnight at 4C. Alexa Fluor 488 detection (green) in combination with nuclear DAPI staining (blue). Data obtained by Ben Jerry Gonzales from Nissim Ben-Arie's lab, Dept. of Cell and Developmental Biology, The Hebrew University of Jeruzalem, Israel.)
Immunofluorescence (IF)
((1ug/ml) staining of PFA-perfused cryosection of Mouse embryo E13.5. Primary incubation overnight at 4C. Alexa Fluor 488 detection (green) in combination with nuclear DAPI staining (blue). Data obtained by Ben Jerry Gonzales from Nissim Ben-Arie's lab, Dept. of Cell and Developmental Biology, The Hebrew University of Jeruzalem, Israel.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,463 Da
NCBI Official Full Name
fer3-like protein
NCBI Official Synonym Full Names
Fer3-like (Drosophila)
NCBI Official Symbol
Ferd3l
NCBI Official Synonym Symbols
Nato; fer3; Nato3; Mnato3; Ntwist; N-twist; bHLHa31
NCBI Protein Information
fer3-like protein
UniProt Protein Name
Fer3-like protein
Protein Family
UniProt Gene Name
Ferd3l
UniProt Synonym Gene Names
Nato; Ntwist
UniProt Entry Name
FER3L_MOUSE
Uniprot Description
FERD3L: Transcription factor that binds to the E-box and functions as inhibitor of transcription. DNA binding requires dimerization with an E protein. Inhibits transcription activation by ASCL1/MASH1 by sequestering E proteins.
Protein type: DNA-binding
Cellular Component: nucleus
Molecular Function: DNA binding; protein dimerization activity
Biological Process: cell development; floor plate development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; regulation of neurogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on Ferd3l
Similar Products
Product Notes
The Ferd3l ferd3l (Catalog #AAA423439) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Ferd3l (aa56-68) Antibody reacts with Tested: Mouse; Expected from sequence similarity: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ferd3l can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: Frozen sections of Mouse embryo E13.5 shows staining of the floor plate of the developing spinal cord. Recommended concentration, 1-3ug/ml. Researchers should empirically determine the suitability of the Ferd3l ferd3l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QFDERYQEVE GDE. It is sometimes possible for the material contained within the vial of "Ferd3l, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
