Goat Prdm9 Polyclonal Antibody | anti-Prdm9 antibody
Goat anti-Prdm9 (mouse, aa440-452) Antibody
Gene Names
Prdm9; Rcr1; Dsbc1; repro7; Meisetz; PRDM9-B; BC012016; G1-419-29
Reactivity
Expected from sequence similarity: Mouse
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Prdm9; Polyclonal Antibody; Goat anti-Prdm9 (mouse; aa440-452) Antibody; PR domain containing 9; RP23-64O5.3; BC012016; Dsbc1; G1-419-29; Meisetz; Rcr1; repro7; PR domain zinc finger protein 9; PR domain-containing protein 9; PR-domain zinc finger protein; VPR domain containing 9; histone-lysine N-methyltransferase PRDM; Prdm9 antibody; PR domain containing 9 antibody; RP23-64O5.3 antibody; BC012016 antibody; Dsbc1 antibody; G1-419-29 antibody; Meisetz antibody; Rcr1 antibody; repro7 antibody; PR domain zinc finger protein 9 antibody; PR domain-containing protein 9 antibody; PR-domain zinc finger protein antibody; VPR domain containing 9 antibody; histone-lysine N-methyltransferase PRDM9 antibody; hybrid sterility protein 1 antibody; meiosis-induced factor containing a PR/SET domain and zinc-finger motif antibody; Prdm9 (mouse; aa440-452); anti-Prdm9 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Mouse
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QEQHVDSQNKNDK
Sequence Length
847
Applicable Applications for anti-Prdm9 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Preliminary experiments gave an approx 70kDa band in Mouse fetal Brain lysates and in lysates of cell line NIH3T3 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.8kDa according to NP_659058.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Preliminary experiments gave an approx 70kDa band in Mouse fetal Brain lysates and in lysates of cell line NIH3T3 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.8kDa according to NP_659058.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-QEQHVDSQNKNDK, from the internal region of the protein sequence according to NP_659058.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,241 Da
NCBI Official Full Name
histone-lysine N-methyltransferase PRDM9
NCBI Official Synonym Full Names
PR domain containing 9
NCBI Official Symbol
Prdm9
NCBI Official Synonym Symbols
Rcr1; Dsbc1; repro7; Meisetz; PRDM9-B; BC012016; G1-419-29
NCBI Protein Information
histone-lysine N-methyltransferase PRDM9
UniProt Protein Name
Histone-lysine N-methyltransferase PRDM9
Protein Family
UniProt Gene Name
Prdm9
UniProt Synonym Gene Names
Hst1; Meisetz
UniProt Entry Name
PRDM9_MOUSE
Uniprot Description
PRDM9: Histone methyltransferase that specifically trimethylates 'Lys-4' of histone H3 during meiotic prophase and is essential for proper meiotic progression. Does not have the ability to mono- and dimethylate 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes during early meiotic prophase.
Protein type: Methyltransferase; Methyltransferase, protein lysine; EC 2.1.1.43; C2H2-type zinc finger protein
Cellular Component: chromosome; intracellular; nucleoplasm; nucleus
Molecular Function: histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; metal ion binding; methyltransferase activity; nucleic acid binding; sequence-specific DNA binding; transferase activity
Biological Process: chromatin modification; histone methylation; meiotic cell cycle; meiotic gene conversion; methylation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; spermatogenesis; transcription, DNA-dependent
Research Articles on Prdm9
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Product Notes
The Prdm9 prdm9 (Catalog #AAA423332) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Prdm9 (mouse, aa440-452) Antibody reacts with Expected from sequence similarity: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Prdm9 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Preliminary experiments gave an approx 70kDa band in Mouse fetal Brain lysates and in lysates of cell line NIH3T3 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.8kDa according to NP_659058.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the Prdm9 prdm9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QEQHVDSQNK NDK. It is sometimes possible for the material contained within the vial of "Prdm9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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