Western Blot (WB)
((0.1ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CD3E Polyclonal Antibody | anti-CD3E antibody
Goat anti-CD3E (cytoplasmic) Antibody
Western Blot: Approx 22kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1kDa according to NP_000724.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Jurkat (A) and Molt4 (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Uniprot Description
CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 11q23
Cellular Component: alpha-beta T cell receptor complex; external side of plasma membrane; immunological synapse; integral to plasma membrane; intercellular junction; plasma membrane; T cell receptor complex
Molecular Function: protein binding; protein heterodimerization activity; protein kinase binding; receptor signaling complex scaffold activity; receptor signaling protein activity; SH3 domain binding; T cell receptor binding; transmembrane receptor activity
Biological Process: cell surface receptor linked signal transduction; G-protein coupled receptor protein signaling pathway; negative regulation of smoothened signaling pathway; negative thymic T cell selection; positive regulation of alpha-beta T cell proliferation; positive regulation of calcium-mediated signaling; positive regulation of interferon-gamma production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of T cell anergy; positive regulation of T cell proliferation; protein complex assembly; regulation of apoptosis; regulation of immune response; response to nutrient; signal complex assembly; T cell activation; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway
Disease: Immunodeficiency 18
Research Articles on CD3E
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Product Notes
The CD3E cd3e (Catalog #AAA423329) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CD3E (cytoplasmic) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's CD3E can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 22kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1kDa according to NP_000724.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the CD3E cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RKGQRDLYSG LNQR. It is sometimes possible for the material contained within the vial of "CD3E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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