Western Blot (WB)
((2ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat EAAT1/GLAST1 Polyclonal Antibody | anti-SLC1A3 antibody
Goat anti-EAAT1/GLAST1 Antibody
Western Blot: Approx 60kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 59.6kDa according to NP_004163.3). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((2ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]
Uniprot Description
SLC1A3: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Mitochondrial; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 5p13
Cellular Component: basolateral plasma membrane; cell soma; cell surface; dendritic spine; fibril; integral to membrane; membrane; mitochondrial inner membrane; plasma membrane
Molecular Function: amino acid transmembrane transporter activity; glutamate binding; high-affinity glutamate transmembrane transporter activity; L-glutamate transmembrane transporter activity; sodium:dicarboxylate symporter activity
Biological Process: auditory behavior; cranial nerve development; gamma-aminobutyric acid biosynthetic process; glutamate biosynthetic process; glutamate secretion; ion transport; L-glutamate import; malate-aspartate shuttle; neuromuscular process controlling balance; neuron morphogenesis during differentiation; neurotransmitter uptake; positive regulation of defense response to virus by host; positive regulation of synaptic transmission; response to antibiotic; response to drug; response to light stimulus; response to wounding; sensory perception of sound; synaptic transmission
Disease: Episodic Ataxia, Type 6
Research Articles on SLC1A3
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Product Notes
The SLC1A3 slc1a3 (Catalog #AAA423166) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EAAT1/GLAST1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's EAAT1/GLAST1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 60kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 59.6kDa according to NP_004163.3). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the SLC1A3 slc1a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DNETEKPIDS ETK. It is sometimes possible for the material contained within the vial of "EAAT1/GLAST1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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