Goat LSM2 Polyclonal Antibody | anti-LSM2 antibody

Goat anti-LSM2 (aa33-46) Antibody

Cat. Num. AAA423147

• Gene Names: LSM2; G7B; snRNP; C6orf28; YBL026W
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: LSM2; Polyclonal Antibody; Goat anti-LSM2 (aa33-46) Antibody; LSM2 homolog; U6 small nuclear RNA associated (S. cerevisiae); C6orf28; G7b; YBL026W; snRNP; OTTHUMP00000029159; U6 snRNA-associated Sm-like protein LSm2; protein G7b; small nuclear ribonuclear protein D homolog; snRNP core Sm-like protein Sm-x5; C6orf28 antibody; G7b antibody; LSM2 antibody; U6 small nuclear RNA associated (S. cerevisiae) antibody; OTTHUMP00000029159 antibody; protein G7b antibody; small nuclear ribonuclear protein D homolog antibody; snRNP antibody; snRNP core Sm-like protein Sm-x5 antibody; U6 snRNA-associated Sm-like protein LSm2 antibody; YBL026W antibody; LSM2 (aa33-46); anti-LSM2 antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DQYLNIKLTDISVT
• Sequence Length: 95

• Applicable Applications for anti-LSM2 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 2000.; Western Blot: Approx 10kDa band observed in lysates of cell line K562 and NIH3T3 (calculated MW of 10kDa according to NP_067000.1). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-DQYLNIKLTDISVT, from the internal region of the protein sequence according to NP_067000.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 10863977
• NCBI GeneID: 57819
• NCBI Accession #: NP_067000.1
• NCBI GenBank Nucleotide #: NM_021177.4
• Molecular Weight: 10,835 Da
• NCBI Official Full Name: U6 snRNA-associated Sm-like protein LSm2
• NCBI Official Synonym Full Names: LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated
• NCBI Official Symbol: LSM2
• NCBI Official Synonym Symbols: G7B; snRNP; C6orf28; YBL026W
• NCBI Protein Information: U6 snRNA-associated Sm-like protein LSm2
• UniProt Protein Name: U6 snRNA-associated Sm-like protein LSm2
• Protein Family: Sm-like protein
• UniProt Gene Name: LSM2
• UniProt Synonym Gene Names: C6orf28; G7B
• UniProt Entry Name: LSM2_HUMAN

NCBI Description

This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 19. [provided by RefSeq, Nov 2011]

Uniprot Description

LSM2: Binds specifically to the 3'-terminal U-tract of U6 snRNA. May be involved in pre-mRNA splicing. Identified in the spliceosome C complex. LSm subunits form a heteromer with a doughnut shape. Belongs to the snRNP Sm proteins family.

Protein type: Spliceosome; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytosol; nucleoplasm; U4/U6 x U5 tri-snRNP complex

Molecular Function: protein binding; Ral GTPase binding; RNA binding; U6 snRNA binding

Biological Process: nuclear mRNA splicing, via spliceosome