Western Blot (WB)
((0.1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat CSTF3 Polyclonal Antibody | anti-CSTF3 antibody
Goat anti-CSTF3 Antibody
Western Blot: Approx 75kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 82.9kDa according to NP_001317.1). The observed molecular weight corresponds to the expected size of 77kDa. Recommended concentration: 0. 03-0.1ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
CstF-77: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: nucleoplasm; nucleus
Molecular Function: mRNA binding; protein binding; RNA binding
Biological Process: mRNA 3'-end processing; mRNA cleavage; mRNA polyadenylation; nuclear mRNA splicing, via spliceosome; termination of RNA polymerase II transcription
Research Articles on CSTF3
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Product Notes
The CSTF3 cstf3 (Catalog #AAA423071) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CSTF3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's CSTF3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 75kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 82.9kDa according to NP_001317.1). The observed molecular weight corresponds to the expected size of 77kDa. Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the CSTF3 cstf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RETKGKLPSY KE. It is sometimes possible for the material contained within the vial of "CSTF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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