Western Blot (WB)
((0. 03ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Fubp1 Polyclonal Antibody | anti-Fubp1 antibody
Goat anti-Fubp1 (mouse, aa160-174) Antibody
Gene Names
Fubp1; FBP; Fubp; Fubp4; D3Ertd330e; 9530027K12Rik
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Fubp1; Polyclonal Antibody; Goat anti-Fubp1 (mouse; aa160-174) Antibody; far upstream element (FUSE) binding protein 1; 9530027K12Rik; D3Ertd330e; FBP; Fubp; Fubp4; FUSE-binding protein 1; far upstream element (FUSE) binding protein 4; far upstream element-binding protein 1; 9530027K12Rik antibody; D3Ertd330e antibody; far upstream element (FUSE) binding protein 1 antibody; far upstream element (FUSE) binding protein 4 antibody; far upstream element-binding protein 1 antibody; FBP antibody; Fubp antibody; Fubp1 antibody; Fubp4 antibody; FUSE-binding protein 1 antibody; Fubp1 (mouse; aa160-174); anti-Fubp1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
DQIVEKGRPAPGFHH
Sequence Length
642
Applicable Applications for anti-Fubp1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Tonsil shows staining in a selection of cells mostly outside the germinal centre. Recommended concentration, 4-8ug/ml.
Western Blot: Approx 75kDa band observed in lysates of cell line Jurkat (calculated MW of 67.4kDa according to NP_476513.2). Recommended concentration: 0. 03-0.1ug/ml.
Immunohistochemistry: In paraffin embedded Human Tonsil shows staining in a selection of cells mostly outside the germinal centre. Recommended concentration, 4-8ug/ml.
Western Blot: Approx 75kDa band observed in lysates of cell line Jurkat (calculated MW of 67.4kDa according to NP_476513.2). Recommended concentration: 0. 03-0.1ug/ml.
Immunogen
Peptide with sequence C-DQIVEKGRPAPGFHH, from the internal region of the protein sequence according to NP_476513.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0. 03ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70,871 Da
NCBI Official Full Name
far upstream element-binding protein 1
NCBI Official Synonym Full Names
far upstream element (FUSE) binding protein 1
NCBI Official Symbol
Fubp1
NCBI Official Synonym Symbols
FBP; Fubp; Fubp4; D3Ertd330e; 9530027K12Rik
NCBI Protein Information
far upstream element-binding protein 1
UniProt Protein Name
Far upstream element-binding protein 1
Protein Family
UniProt Gene Name
Fubp1
UniProt Synonym Gene Names
D3Ertd330e; FBP; FUSE-binding protein 1
UniProt Entry Name
FUBP1_MOUSE
Uniprot Description
FBP1: Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA splicing; Motility/polarity/chemotaxis; DNA-binding; RNA-binding; Transcription factor
Cellular Component: nucleoplasm; nucleus
Molecular Function: DNA binding; nucleic acid binding; protein binding; RNA binding
Biological Process: cell growth; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; stem cell division; transcription, DNA-dependent
Research Articles on Fubp1
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Product Notes
The Fubp1 fubp1 (Catalog #AAA422947) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Fubp1 (mouse, aa160-174) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Fubp1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Tonsil shows staining in a selection of cells mostly outside the germinal centre. Recommended concentration, 4-8ug/ml. Western Blot: Approx 75kDa band observed in lysates of cell line Jurkat (calculated MW of 67.4kDa according to NP_476513.2). Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the Fubp1 fubp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DQIVEKGRPA PGFHH. It is sometimes possible for the material contained within the vial of "Fubp1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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