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Western Blot (WB) ((0. 03ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat PLA2G4C Polyclonal Antibody | anti-PLA2G4C antibody

Goat anti-PLA2G4C Antibody

Gene Names
PLA2G4C; CPLA2-gamma
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PLA2G4C; Polyclonal Antibody; Goat anti-PLA2G4C Antibody; phospholipase A2; group IVC (cytosolic; calcium-independent); CPLA2-gamma; DKFZp586C0423; FLJ42247; FLJ44164; cytosolic phospholipase A2 gamma; cytosolic; calcium-independent; gamma; group IVC; CPLA2-gamma antibody; cytosolic phospholipase A2 gamma antibody; DKFZp586C0423 antibody; FLJ42247 antibody; FLJ44164 antibody; gamma antibody; group IVC antibody; calcium-independent) antibody; PLA2G4C antibody; anti-PLA2G4C antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
NTEVIREYIFDQLR
Sequence Length
541
Applicable Applications for anti-PLA2G4C antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 65kDa band observed in Human Brain (Hippocampus), Liver, Pancreas, and Spleen lysates (calculated MW of 62.5kDa according to NP_001152794.1). Recommended concentration: 0. 03-0.1ug/ml.
Immunogen
Peptide with sequence C-NTEVIREYIFDQLR, from the internal region of the protein sequence according to NP_003697.2; NP_001152794.1; NP_001152795.1.
Epitope
Internal region
Note
This antibody is expected to recognize all reported isoforms (NP_003697.2; NP_001152794.1; NP_001152795.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0. 03ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0. 03ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
Molecular Weight
62,451 Da
NCBI Official Full Name
cytosolic phospholipase A2 gamma isoform 1
NCBI Official Synonym Full Names
phospholipase A2 group IVC
NCBI Official Symbol
PLA2G4C
NCBI Official Synonym Symbols
CPLA2-gamma
NCBI Protein Information
cytosolic phospholipase A2 gamma
UniProt Protein Name
Cytosolic phospholipase A2 gamma
Protein Family
UniProt Gene Name
PLA2G4C
UniProt Synonym Gene Names
cPLA2-gamma
UniProt Entry Name
PA24C_HUMAN

NCBI Description

This gene encodes a protein which is a member of the phospholipase A2 enzyme family which hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids, both of which serve as precursors in the production of signaling molecules. The encoded protein has been shown to be a calcium-independent and membrane bound enzyme. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]

Uniprot Description

PLA2G4C: a calcium-independent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Has a preference for arachidonic acid at the sn-2 position of phosphatidylcholine as compared with palmitic acid. Highly expressed in heart and skeletal muscle.

Protein type: Phospholipase; Lipid Metabolism - arachidonic acid; EC 3.1.1.4

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cell cortex; cytosol; endoplasmic reticulum membrane; membrane; nuclear envelope; nucleoplasm

Molecular Function: calcium-independent phospholipase A2 activity; lysophospholipase activity; phospholipase A1 activity; phospholipase A2 activity; phospholipid binding

Biological Process: arachidonic acid metabolic process; glycerophospholipid catabolic process; inflammatory response; parturition; phospholipid metabolic process

Research Articles on PLA2G4C

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Product Notes

The PLA2G4C pla2g4c (Catalog #AAA422936) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PLA2G4C Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G4C can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 65kDa band observed in Human Brain (Hippocampus), Liver, Pancreas, and Spleen lysates (calculated MW of 62.5kDa according to NP_001152794.1). Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the PLA2G4C pla2g4c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NTEVIREYIF DQLR. It is sometimes possible for the material contained within the vial of "PLA2G4C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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