Western Blot (WB)
((0. 01ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat AP2A1 Polyclonal Antibody | anti-AP2A1 antibody
Goat anti-AP2A1 (aa706-727) Antibody
Western Blot: Approx 110kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kDa according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03ug/ml.
Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane.
Western Blot (WB)
((0. 01ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunofluorescence (IF)
((0.1ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK)
Immunofluorescence (IF)
((0.1ug/ml) staining of methanol-fixed HeLa cells with stably expressing Mouse Ap2a1. Primary incubation was 1 hour. Detected by Alexa Fluor 594. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK)
Testing Data
((0.1ug/ml) staining of Mouse Brain lysate (~5ug protein in SDSPAGE buffer). The left panel shows the same blot stained with Ponceau red for total protein stain as the loading control before labelling. Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK)
Testing Data
((0.1ug/ml) staining of Mouse Brain lysate (~5ug protein in SDSPAGE buffer). The left panel shows the same blot stained with Ponceau red for total protein stain as the loading control before labelling. Primary incubation was 1 hour. Detected by chemiluminescence. Data obtained from Prof. M Robinson, CIMR, Cambridge, UK)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
AP2A1: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C- terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Belongs to the adaptor complexes large subunit family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Vesicle
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: AP-2 adaptor complex; apical plasma membrane; basolateral plasma membrane; clathrin coat of trans-Golgi network vesicle; cytosol; filopodium tip; membrane; plasma membrane
Molecular Function: protein binding; protein C-terminus binding; protein complex binding; protein kinase binding; protein transporter activity
Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; endocytosis; ephrin receptor signaling pathway; Golgi to endosome transport; intracellular protein transport; microtubule-based movement; negative regulation of epidermal growth factor receptor signaling pathway; regulation of defense response to virus by virus; Wnt receptor signaling pathway, planar cell polarity pathway
Research Articles on AP2A1
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Product Notes
The AP2A1 ap2a1 (Catalog #AAA422900) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-AP2A1 (aa706-727) Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's AP2A1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 110kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 108kDa according to NP_055018.2). The same band is observed in Mouse Brain, but not in Mouse Liver or Mouse Kidney lysates. Recommended concentration: 0.01-0.03ug/ml. Immunocytochemistry: HeLa stably expressing a chimeric construct including the brain-specific splice variant of Mouse Ap2a1, showing clathrin-coated pits and vesicles budding from the plasma membrane. Researchers should empirically determine the suitability of the AP2A1 ap2a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ELEPPAPESP MALLADPAPA AD. It is sometimes possible for the material contained within the vial of "AP2A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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