Goat Npc1l1 Polyclonal Antibody | anti-Npc1l1 antibody

Goat anti-Npc1l1 (mouse) Antibody

Cat. Num. AAA422795

• Gene Names: Npc1l1; Gm243; 9130221N23Rik
• Reactivity: Expected from sequence similarity: Mouse, Rat
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Npc1l1; Polyclonal Antibody; Goat anti-Npc1l1 (mouse) Antibody; NPC1-like 1; RP23-198N14.2; 9130221N23Rik; Gm243; Niemann-Pick C1-like protein 1; Niemann-Pick disease; type C1; type C1-like 1; OTTMUSP00000000725; Npc1l1 antibody; NPC1-like 1 antibody; RP23-198N14.2 antibody; 9130221N23Rik antibody; Gm243 antibody; Niemann-Pick C1-like protein 1 antibody; type C1 antibody; type C1-like 1 antibody; OTTMUSP00000000725 antibody; Npc1l1 (mouse); anti-Npc1l1 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SEPSCPQYPFPAD
• Sequence Length: 1333

• Applicable Applications for anti-Npc1l1 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Preliminary experiments in Mouse and Rat Liver and Lung lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
• Immunogen: Peptide with sequence SEPSCPQYPFPAD, from the C Terminus (near) of the protein sequence according to NP_997125.2.
• Epitope: C Terminus (near)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 253970442
• NCBI GeneID: 237636
• NCBI Accession #: NP_997125.2
• NCBI GenBank Nucleotide #: NM_207242.2
• Molecular Weight: 147,132 Da
• NCBI Official Full Name: Niemann-Pick C1-like protein 1
• NCBI Official Synonym Full Names: NPC1-like 1
• NCBI Official Symbol: Npc1l1
• NCBI Official Synonym Symbols: Gm243; 9130221N23Rik
• NCBI Protein Information: Niemann-Pick C1-like protein 1
• UniProt Protein Name: Niemann-Pick C1-like protein 1
• Protein Family: Niemann-Pick C1-like protein
• UniProt Gene Name: Npc1l1
• UniProt Entry Name: NPCL1_MOUSE

Uniprot Description

NPC1L1: Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Interacts with RAB11A, MYO5B and RAB11FIP2. Interaction with RAB11A, MYO5B and RAB11FIP2 is required for proper transport to the plasma membrane upon cholesterol depletion. Expression is decreased in Caco-2 cells upon PPARD activation. Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. Belongs to the patched family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: brush border membrane; integral to membrane; membrane; plasma membrane

Molecular Function: drug binding; hedgehog receptor activity; lipid transporter activity; myosin V binding; Rab GTPase binding

Biological Process: cholesterol absorption; cholesterol biosynthetic process; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; lipid metabolic process; lipoprotein metabolic process; steroid metabolic process