Western Blot (WB)
((1ug/ml) staining of nuclear NIH3T3 lysate (A) and of Mouse (B) and Rat (C) Colon lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat IRF2BP1 Polyclonal Antibody | anti-IRF2BP1 antibody
Goat anti-IRF2BP1 (aa461-474) Antibody
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
IRF2BP1; Polyclonal Antibody; Goat anti-IRF2BP1 (aa461-474) Antibody; Goat Anti-IRF2BP1 (aa109-122) Antibody; interferon regulatory factor 2 binding protein 1; DKFZp434M154; IRF2BP1 antibody; interferon regulatory factor 2 binding protein 1 antibody; DKFZp434M154 antibody; IRF2BP1 (aa461-474); anti-IRF2BP1 antibody
Host
Goat
Reactivity
Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QPPTQHRLVARNGE
Sequence Length
584
Applicable Applications for anti-IRF2BP1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.7kDa according to NP_056464.1). An approx 85kDa band observed in nuclear lysates of cell line NIH3T3 and 75kDa in Mouse and Rat Colion lysates. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.7kDa according to NP_056464.1). An approx 85kDa band observed in nuclear lysates of cell line NIH3T3 and 75kDa in Mouse and Rat Colion lysates. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-QPPTQHRLVARNGE, from the internal region of the protein sequence according to NP_056464.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((1ug/ml) staining of nuclear NIH3T3 lysate (A) and of Mouse (B) and Rat (C) Colon lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((1ug/ml) staining of nuclear NIH3T3 lysate (A) and of Mouse (B) and Rat (C) Colon lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,688 Da
NCBI Official Full Name
interferon regulatory factor 2-binding protein 1
NCBI Official Synonym Full Names
interferon regulatory factor 2 binding protein 1
NCBI Official Symbol
IRF2BP1
NCBI Protein Information
interferon regulatory factor 2-binding protein 1
UniProt Protein Name
Interferon regulatory factor 2-binding protein 1
Protein Family
UniProt Gene Name
IRF2BP1
UniProt Synonym Gene Names
IRF-2-binding protein 1; IRF-2BP1
UniProt Entry Name
I2BP1_HUMAN
Uniprot Description
IRF2BP1: Acts as a transcriptional corepressor in a IRF2- dependent manner; this repression is not mediated by histone deacetylase activities. May act as an E3 ligase towards JDP2, enhancing its polyubiquitination. Represses ATF2-dependent transcriptional activation. Belongs to the IRF2BP family.
Protein type: EC 6.3.2.-; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: nucleoplasm
Molecular Function: ligase activity; metal ion binding; protein binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on IRF2BP1
Similar Products
Product Notes
The IRF2BP1 irf2bp1 (Catalog #AAA422604) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-IRF2BP1 (aa461-474) Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's IRF2BP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.7kDa according to NP_056464.1). An approx 85kDa band observed in nuclear lysates of cell line NIH3T3 and 75kDa in Mouse and Rat Colion lysates. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the IRF2BP1 irf2bp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QPPTQHRLVA RNGE. It is sometimes possible for the material contained within the vial of "IRF2BP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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