Western Blot (WB)
((0. 03ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat EIF3E Polyclonal Antibody | anti-EIF3E antibody
Goat anti-EIF3E Antibody
Gene Names
EIF3E; INT6; EIF3S6; EIF3-P48; eIF3-p46
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Cow, Pig
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
EIF3E; Polyclonal Antibody; Goat anti-EIF3E Antibody; eukaryotic translation initiation factor 3; subunit E; EIF3-P48; EIF3S6; INT6; eIF3-p46; subunit 6 (48kD); subunit 6 48kDa; mammary tumor-associated protein INT; eIF3-p46 antibody; EIF3-P48 antibody; EIF3S6 antibody; subunit 6 (48kD) antibody; subunit 6 48kDa antibody; subunit E antibody; INT6 antibody; mammary tumor-associated protein INT antibody; EIF3E antibody; anti-EIF3E antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Cow, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KLNQNSRSEAPN
Sequence Length
445
Applicable Applications for anti-EIF3E antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 50kDa band observed in lysates of cell line Daudi (calculated MW of 52.2kDa according to NP_001559.1). Recommended concentration: 0. 03-0.1ug/ml.
Western Blot: Approx 50kDa band observed in lysates of cell line Daudi (calculated MW of 52.2kDa according to NP_001559.1). Recommended concentration: 0. 03-0.1ug/ml.
Immunogen
Peptide with sequence C-KLNQNSRSEAPN, from the C Terminus of the protein sequence according to NP_001559.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0. 03ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,221 Da
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit E
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3 subunit E
NCBI Official Symbol
EIF3E
NCBI Official Synonym Symbols
INT6; EIF3S6; EIF3-P48; eIF3-p46
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit E
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit E
UniProt Gene Name
EIF3E
UniProt Synonym Gene Names
eIF3e
UniProt Entry Name
EIF3E_HUMAN
Uniprot Description
EIF3E: eukaryotic translation initiation factor 3 subunit 2. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits. Phosphorylated by TGF-beta type II receptor.
Protein type: Translation; Translation initiation
Chromosomal Location of Human Ortholog: 8q22-q23
Cellular Component: chromatin; cytoplasm; cytosol; eukaryotic translation initiation factor 3 complex; membrane; nucleoplasm; nucleus; PML body
Molecular Function: protein binding; protein N-terminus binding; translation initiation factor activity
Biological Process: formation of translation preinitiation complex; mRNA catabolic process, nonsense-mediated decay; negative regulation of translational initiation; regulation of translational initiation; translational initiation
Research Articles on EIF3E
Similar Products
Product Notes
The EIF3E eif3e (Catalog #AAA422496) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EIF3E Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog, Cow, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3E can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx 50kDa band observed in lysates of cell line Daudi (calculated MW of 52.2kDa according to NP_001559.1). Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the EIF3E eif3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KLNQNSRSEA PN. It is sometimes possible for the material contained within the vial of "EIF3E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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