Western Blot (WB)
((0. 01ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat EFNA1 Polyclonal Antibody | anti-EFNA1 antibody
Goat anti-EFNA1 Antibody
Western Blot: Approx 26kDa band observed in Mouse Thymus and Rat Lung lysates (calculated MW of 23.8kDa according to Mouse NP_034237.3 and Rat NP_446051.2). Recommended concentration: 0.01-0.03ug/ml.
Western Blot (WB)
((0. 01ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008]
Uniprot Description
EFNA1: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis. Belongs to the ephrin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Tumor suppressor; Cell development/differentiation; Membrane protein, GPI anchor; Ligand, receptor tyrosine kinase
Chromosomal Location of Human Ortholog: 1q21-q22
Cellular Component: anchored to plasma membrane; extracellular region; integral to plasma membrane; plasma membrane
Molecular Function: ephrin receptor binding; protein binding; receptor binding
Biological Process: activation of MAPK activity; angiogenesis; axon guidance; cell migration; cell-cell signaling; ephrin receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; notochord formation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of angiogenesis; regulation of axonogenesis; regulation of blood vessel endothelial cell migration; regulation of cell adhesion mediated by integrin; regulation of peptidyl-tyrosine phosphorylation
Research Articles on EFNA1
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Product Notes
The EFNA1 efna1 (Catalog #AAA422490) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EFNA1 Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's EFNA1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 26kDa band observed in Mouse Thymus and Rat Lung lysates (calculated MW of 23.8kDa according to Mouse NP_034237.3 and Rat NP_446051.2). Recommended concentration: 0.01-0.03ug/ml. Researchers should empirically determine the suitability of the EFNA1 efna1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KVTVSGKITH SP. It is sometimes possible for the material contained within the vial of "EFNA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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