Goat POU3F3/BRN1/OCT8 Polyclonal Antibody | anti-POU3F3 antibody

Goat anti-POU3F3/BRN1/OCT8 Antibody

Cat. Num. AAA422364

• Gene Names: POU3F3; BRN1; OTF8; oct-8; brain-1
• Reactivity: Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: POU3F3/BRN1/OCT8; Polyclonal Antibody; Goat anti-POU3F3/BRN1/OCT8 Antibody; POU3F3; POU class 3 homeobox 3; BRN1; OTF8; OTF-8; POU domain; class 3; transcription factor 3; brain-1; brain-specific homeobox/POU domain protein 1; brn-1; oct-8; octamer-binding protein 8; octamer-binding transcription factor 8; Brain-1 antibody; BRN1 antibody; OTF8 antibody; POU class 3 homeobox 3 antibody; transcription factor 3 antibody; POU3F3 antibody; OTF-8 antibody; brain-specific homeobox/POU domain protein 1 antibody; brn-1 antibody; oct-8 antibody; octamer-binding protein 8 antibody; octamer-binding transcription factor 8 antibody; POU3F3 / BRN1 / OCT8; anti-POU3F3 antibody

• Host: Goat
• Reactivity: Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: HMLSHAHQWVTAL
• Sequence Length: 500

• Applicable Applications for anti-POU3F3 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 4000.; Western Blot: Approx 48kDa band observed in Mouse and Rat Spinal Cord lysates (calculated MW of 50.3kDa according to Human NP_006227.1 and 50.2kDa according to Mouse NP_032926.2). Recommended concentration: 1-3ug/ml.
• Immunogen: Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Mouse Spinal Cord lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 5453936
• NCBI GeneID: 5455
• NCBI Accession #: NP_006227.1
• NCBI GenBank Nucleotide #: NM_006236.2
• NCBI Official Full Name: POU domain, class 3, transcription factor 3
• NCBI Official Synonym Full Names: POU class 3 homeobox 3
• NCBI Official Symbol: POU3F3
• NCBI Official Synonym Symbols: BRN1; OTF8; oct-8; brain-1
• NCBI Protein Information: POU domain, class 3, transcription factor 3
• UniProt Protein Name: POU domain, class 3, transcription factor 3
• Protein Family: POU domain, class 3, transcription factor
• UniProt Gene Name: POU3F3
• UniProt Synonym Gene Names: BRN1; OTF8; Brain-1; Brn-1; Oct-8; OTF-8
• UniProt Entry Name: PO3F3_HUMAN

NCBI Description

This gene encodes a POU-domain containing protein that functions as a transcription factor. The encoded protein recognizes an octamer sequence in the DNA of target genes. This protein may play a role in development of the nervous system. [provided by RefSeq, Apr 2015]

Uniprot Description

Oct8: Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met- mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3'). Belongs to the POU transcription factor family. Class- 3 subfamily.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q12.1

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: central nervous system development; cerebral cortex radially oriented cell migration; forebrain ventricular zone progenitor cell division; negative regulation of apoptosis; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription, DNA-dependent