Goat USP43 Polyclonal Antibody | anti-USP43 antibody

Goat anti-USP43 Antibody

Cat. Num. AAA422242

• Reactivity: Expected from sequence similarity: Human
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: USP43; Polyclonal Antibody; Goat anti-USP43 Antibody; ubiquitin specific peptidase 43; FLJ30626; ubiquitin specific protease 43; USP43 antibody; ubiquitin specific peptidase 43 antibody; FLJ30626 antibody; ubiquitin specific protease 43 antibody; anti-USP43 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: SQRTVPGEQASYGT
• Sequence Length: 1123

• Applicable Applications for anti-USP43 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Preliminary experiments gave an approx 170kDa band in Human Testis lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 123kDa according to NP_694942.3. The 170kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-SQRTVPGEQASYGT, from the internal region of the protein sequence according to NP_694942.3.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 148612811
• NCBI GeneID: 124739
• NCBI Accession #: NP_694942.3
• NCBI GenBank Nucleotide #: NM_153210.4
• Molecular Weight: 122,294 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 43 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 43
• NCBI Official Symbol: USP43
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 43
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 43
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: USP43
• UniProt Entry Name: UBP43_HUMAN

Uniprot Description

USP43: May recognize and hydrolyze the peptide bond at the C- terminal Gly of ubiquitin. Involved in the processing of poly- ubiquitin precursors as well as that of ubiquitinated proteins. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Protease

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm

Molecular Function: ISG15-specific protease activity

Biological Process: bypass DNA synthesis; protein deubiquitination; ubiquitin-dependent protein catabolic process