Goat CDKAL1 Polyclonal Antibody | anti-CDKAL1 antibody
Goat anti-CDKAL1 Antibody
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
CDKAL1; Polyclonal Antibody; Goat anti-CDKAL1 Antibody; CDK5 regulatory subunit associated protein 1-like 1; FLJ20342; FLJ46705; MGC75469; CDK5 regulatory subunit associated protein 1-like 1 antibody; FLJ20342 antibody; FLJ46705 antibody; MGC75469 antibody; anti-CDKAL1 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QVKKQRTKDLSR
Sequence Length
579
Applicable Applications for anti-CDKAL1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: This antibody has been described in a publication and it has been successfully used on Human islets and pancreatic insulinoma INS-1 and MIN6 cells. Preliminary experiments gave an approx.39kDa band in Human Brain (Cerebellum) lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 65.1kDa according to NP_060244.2. The 39kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: This antibody has been described in a publication and it has been successfully used on Human islets and pancreatic insulinoma INS-1 and MIN6 cells. Preliminary experiments gave an approx.39kDa band in Human Brain (Cerebellum) lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 65.1kDa according to NP_060244.2. The 39kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-QVKKQRTKDLSR, from the internal region of the protein sequence according to NP_060244.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,043 Da
NCBI Official Full Name
threonylcarbamoyladenosine tRNA methylthiotransferase
NCBI Official Synonym Full Names
CDK5 regulatory subunit associated protein 1 like 1
NCBI Official Symbol
CDKAL1
NCBI Protein Information
threonylcarbamoyladenosine tRNA methylthiotransferase
UniProt Protein Name
Threonylcarbamoyladenosine tRNA methylthiotransferase
Protein Family
UniProt Gene Name
CDKAL1
UniProt Entry Name
CDKAL_HUMAN
NCBI Description
The protein encoded by this gene is a member of the methylthiotransferase family. The function of this gene is not known. Genome-wide association studies have linked single nucleotide polymorphisms in an intron of this gene with susceptibilty to type 2 diabetes. [provided by RefSeq, May 2010]
Uniprot Description
CDKAL1: Catalyzes the methylthiolation of N6- threonylcarbamoyladenosine (t(6)A), leading to the formation of 2- methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine. Belongs to the methylthiotransferase family. CDKAL1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Enzyme, misc.; EC 2.8.4.5; Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p22.3
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane; rough endoplasmic reticulum
Molecular Function: 4 iron, 4 sulfur cluster binding; metal ion binding; protein binding
Biological Process: tRNA modification
Disease: Diabetes Mellitus, Noninsulin-dependent
Research Articles on CDKAL1
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Product Notes
The CDKAL1 cdkal1 (Catalog #AAA422147) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CDKAL1 Antibody reacts with Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's CDKAL1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: This antibody has been described in a publication and it has been successfully used on Human islets and pancreatic insulinoma INS-1 and MIN6 cells. Preliminary experiments gave an approx.39kDa band in Human Brain (Cerebellum) lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 65.1kDa according to NP_060244.2. The 39kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the CDKAL1 cdkal1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QVKKQRTKDL SR. It is sometimes possible for the material contained within the vial of "CDKAL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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