Western Blot (WB)
((0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat SH2D3A/NSP1 Polyclonal Antibody | anti-SH2D3A antibody
Goat anti-SH2D3A/NSP1 Antibody
Gene Names
SH2D3A; NSP1
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
SH2D3A/NSP1; Polyclonal Antibody; Goat anti-SH2D3A/NSP1 Antibody; SH2D3A; NSP1; SH2 domain containing 3A; SH2 domain-containing 3A; novel SH2-containing protein 1; SH2D3A antibody; NSP1 antibody; SH2 domain containing 3A antibody; SH2 domain-containing 3A antibody; novel SH2-containing protein 1 antibody; SH2D3A / NSP1; anti-SH2D3A antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
APRAERFEKFQR
Sequence Length
576
Applicable Applications for anti-SH2D3A antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx. 60kDa band observed in Human Lung and Tonsil lysates (calculated MW of 63.1kDa according to NP_005481.2). Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx. 60kDa band observed in Human Lung and Tonsil lysates (calculated MW of 63.1kDa according to NP_005481.2). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-APRAERFEKFQR, from the internal region (near C terminus) of the protein sequence according to NP_005481.2.
Epitope
Internal region (near C Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,402 Da
NCBI Official Full Name
SH2 domain-containing protein 3A
NCBI Official Synonym Full Names
SH2 domain containing 3A
NCBI Official Symbol
SH2D3A
NCBI Official Synonym Symbols
NSP1
NCBI Protein Information
SH2 domain-containing protein 3A
UniProt Protein Name
SH2 domain-containing protein 3A
Protein Family
UniProt Gene Name
SH2D3A
UniProt Synonym Gene Names
NSP1
UniProt Entry Name
SH23A_HUMAN
Uniprot Description
SH2D3A: May play a role in JNK activation.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: intracellular
Molecular Function: guanyl-nucleotide exchange factor activity; protein binding; SH3/SH2 adaptor activity
Biological Process: JNK cascade; positive regulation of GTPase activity; positive regulation of signal transduction; small GTPase mediated signal transduction
Research Articles on SH2D3A
Similar Products
Product Notes
The SH2D3A sh2d3a (Catalog #AAA421973) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SH2D3A/NSP1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's SH2D3A/NSP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx. 60kDa band observed in Human Lung and Tonsil lysates (calculated MW of 63.1kDa according to NP_005481.2). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the SH2D3A sh2d3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: APRAERFEKF QR. It is sometimes possible for the material contained within the vial of "SH2D3A/NSP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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