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Western Blot (WB) ((0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat XPNPEP1 Polyclonal Antibody | anti-XPNPEP1 antibody

Goat anti-XPNPEP1 Antibody

Gene Names
XPNPEP1; APP1; SAMP; XPNPEP; XPNPEPL; XPNPEPL1
Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
XPNPEP1; Polyclonal Antibody; Goat anti-XPNPEP1 Antibody; X-prolyl aminopeptidase (aminopeptidase P) 1; soluble; SAMP; XPNPEP; XPNPEPL; XPNPEPL1; OTTHUMP00000020457; OTTHUMP00000058856; soluble (SAMP; XPNPEPL); X-prolyl aminopeptidase (aminopeptidase; XPNPEP1 antibody; soluble antibody; SAMP antibody; XPNPEP antibody; XPNPEPL antibody; XPNPEPL1 antibody; OTTHUMP00000020457 antibody; OTTHUMP00000058856 antibody; XPNPEPL) antibody; X-prolyl aminopeptidase (aminopeptidase P)-like antibody; anti-XPNPEP1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
LIRETQPISKQH
Sequence Length
623
Applicable Applications for anti-XPNPEP1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 80kDa band observed in Human Heart, Skeletal Muscle and Pancreas lysates and 75kDa band observed in Mouse and Rat Small Intestines (calculated MW of 69.9kDa according to NP_065116.2). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-LIRETQPISKQH, from the C Terminus of the protein sequence according to NP_065116.2; NP_001161076.1.
Epitope
C Terminus
Note
This antibody is expected to recognize isoform 1 and 2 (NP_065116.2; NP_001161076.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0.1ug/ml) staining of Mouse (A) and Rat (B) Small Intestine lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of Mouse (A) and Rat (B) Small Intestine lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

((0.1ug/ml) staining of Pig Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of Pig Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72,107 Da
NCBI Official Full Name
X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
NCBI Official Synonym Full Names
X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
NCBI Official Symbol
XPNPEP1
NCBI Official Synonym Symbols
APP1; SAMP; XPNPEP; XPNPEPL; XPNPEPL1
NCBI Protein Information
xaa-Pro aminopeptidase 1
UniProt Protein Name
Xaa-Pro aminopeptidase 1
UniProt Gene Name
XPNPEP1
UniProt Synonym Gene Names
sAmp
UniProt Entry Name
XPP1_HUMAN

NCBI Description

This gene encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]

Uniprot Description

XPNPEP1: Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. Belongs to the peptidase M24B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.11.9

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: cytoplasm

Molecular Function: aminopeptidase activity; manganese ion binding; protein homodimerization activity

Biological Process: proteolysis

Research Articles on XPNPEP1

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Product Notes

The XPNPEP1 xpnpep1 (Catalog #AAA421959) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-XPNPEP1 Antibody reacts with Tested: Human, Mouse, Rat, Pig; Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's XPNPEP1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 80kDa band observed in Human Heart, Skeletal Muscle and Pancreas lysates and 75kDa band observed in Mouse and Rat Small Intestines (calculated MW of 69.9kDa according to NP_065116.2). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the XPNPEP1 xpnpep1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LIRETQPISK QH. It is sometimes possible for the material contained within the vial of "XPNPEP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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