Western Blot (WB)
((1ug/ml) staining of untreated (first lane) and TGF-bl-treated (second lane) primary cultured Human Lung fibroblast lysate. Primary incubated overnight at 4C. Detected by western blot using chemiluminescence. Data kindly provided by Nickolai Dulin, Dept Medicine/Pulmonary, Chicago, USA.)
Goat SRF Polyclonal Antibody | anti-SRF antibody
Goat anti-SRF Antibody
Western Blot: Approx 67kDa band observed in primary cultured Human Lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Davis FJ et al, Am J Physiol Heart Circ Physiol.2002 Apr;282(4): H1521-33.; PMID: 11893590). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((1ug/ml) staining of untreated (first lane) and TGF-bl-treated (second lane) primary cultured Human Lung fibroblast lysate. Primary incubated overnight at 4C. Detected by western blot using chemiluminescence. Data kindly provided by Nickolai Dulin, Dept Medicine/Pulmonary, Chicago, USA.)
Western Blot (WB)
((1ug/ml) staining of untreated (first lane) and TGF-bl-treated (second lane) primary cultured Human Lung fibroblast lysate. Primary incubated overnight at 4C. Detected by western blot using chemiluminescence. Data kindly provided by Nickolai Dulin, Dept Medicine/Pulmonary, Chicago, USA.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
Uniprot Description
SRF: a transcription factor of the MADS domain family that binds to the serum response element (SRE). Regulates the transcription of immediate early genes including c-fos. Binds DNA as a multimer, probably a dimer.
Protein type: Transcription factor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6p21.1
Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus
Molecular Function: chromatin DNA binding; histone deacetylase binding; protein binding; protein homodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity; transcription factor binding
Biological Process: angiogenesis involved in wound healing; associative learning; cardiac myofibril assembly; cell migration during sprouting angiogenesis; cell-matrix adhesion; developmental growth; erythrocyte development; heart development; heart looping; hippocampus development; long-term memory; mesoderm formation; morphogenesis of an epithelial sheet; mRNA transcription from RNA polymerase II promoter; muscle maintenance; negative regulation of cell migration; negative regulation of cell proliferation; neurite development; neuron development; neuron migration; patterning of blood vessels; platelet activation; platelet formation; positive regulation of axon extension; positive regulation of cell differentiation; positive regulation of filopodium formation; positive regulation of smooth muscle contraction; positive regulation of transcription by glucose; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive thymic T cell selection; regulation of cell adhesion; regulation of smooth muscle cell differentiation; regulation of water loss via skin; response to cytokine stimulus; response to hormone stimulus; response to hypoxia; response to toxin; sarcomere organization; skin morphogenesis; stress fiber formation; tangential migration from the subventricular zone to the olfactory bulb; thymus development; thyroid gland development; transcription from RNA polymerase II promoter; trophectodermal cell differentiation
Research Articles on SRF
Similar Products
Product Notes
The SRF srf (Catalog #AAA421850) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SRF Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's SRF can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 67kDa band observed in primary cultured Human Lung fibroblasts lysates after 24 h treatment with 1ng/ml TGF-bl (calculated MW of 51.6kDa according to NP_003122.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Davis FJ et al, Am J Physiol Heart Circ Physiol.2002 Apr;282(4): H1521-33.; PMID: 11893590). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the SRF srf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QASPSRDSST DLTQ. It is sometimes possible for the material contained within the vial of "SRF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
