Goat TRPM8 Antibody | anti-TRPM8 antibody

Goat anti-TRPM8 Antibody

Cat. Num. AAA421789

• Gene Names: TRPM8; TRPP8; LTRPC6
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TRPM8; Goat anti-TRPM8 Antibody; transient receptor potential cation channel; subfamily M; member 8; LTRPC6; MGC2849; TRPP8; short form of the TRPM8 cationic channel; transient receptor potential subfamily M member 8; anti-TRPM8 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

• Applicable Applications for anti-TRPM8 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000.; Western blot: Preliminary experiments gave an approx 28kDa band in lysates of cell line NIH3T3 after 1ug/ml antibody staining. ; Please note that currently we cannot find an explanation in the literature for the band we observe given the alculated size of 128kDa according to NP_076985.4.; The 28kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-TSEQDKDNWNGQLK, from the internal region of the protein sequence according to NP_076985.4.
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 109689695
• NCBI GeneID: 79054
• NCBI Accession #: NP_076985.4
• NCBI GenBank Nucleotide #: NM_024080.4
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 8
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily M member 8
• NCBI Official Symbol: TRPM8
• NCBI Official Synonym Symbols: TRPP8; LTRPC6
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 8
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 8
• Protein Family: TRPM8 channel-associated factor
• UniProt Gene Name: TRPM8
• UniProt Synonym Gene Names: LTRPC6; TRPP8; LTrpC-6; LTrpC6; Trp-p8
• UniProt Entry Name: TRPM8_HUMAN

Uniprot Description

TRPM8: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, cation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: endoplasmic reticulum membrane; external side of plasma membrane; integral to membrane; lipid raft; plasma membrane

Molecular Function: calcium channel activity; protein binding; protein homodimerization activity

Biological Process: cellular calcium ion homeostasis; detection of temperature stimulus; protein homotetramerization; response to cold; thermoception