Goat TAF8/TAFII43 Polyclonal Antibody | anti-TAF8 antibody

Goat anti-TAF8/TAFII43 Antibody

Cat. Num. AAA421722

• Gene Names: TAF8; II; TAF; TBN; TAFII43; TAFII-43
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TAF8/TAFII43; Polyclonal Antibody; Goat anti-TAF8/TAFII43 Antibody; TAF8; TAF8 RNA polymerase II; TATA box binding protein (TBP)-associated factor; 43kDa; 43; FLJ32821; II; TAF; TAFII43; TBN; OTTHUMP00000016392; TAF(II)43; 50 kD; TATA box binding pr; TAF8 antibody; 43kDa antibody; 43 antibody; FLJ32821 antibody; II antibody; TAF antibody; TAFII43 antibody; TBN antibody; OTTHUMP00000016392 antibody; TAF(II)43 antibody; 50 kD antibody; RNA polymerase II; A; 45/50kDa antibody; TBP-associated factor 8 antibody; TBP-associated factor TAFII43 antibody; TBP-associated factor; 43 kD antibody; taube nuss homolog antibody; TAF8 / TAFII43; anti-TAF8 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: KSYCEHTARTQPT
• Sequence Length: 310

• Applicable Applications for anti-TAF8 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 16000.; Western Blot: Preliminary experiments gave an approx 38kDa band in Human Brain (Amygdala, Cerebellum, Hippocampus) lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 34.3kDa according to NP_612639.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence KSYCEHTARTQPT, from the internal region of the protein sequence according to NP_612639.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 104485446
• NCBI GeneID: 129685
• NCBI Accession #: NP_612639.2
• NCBI GenBank Nucleotide #: NM_138572.2
• Molecular Weight: 37,411 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 8
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor 8
• NCBI Official Symbol: TAF8
• NCBI Official Synonym Symbols: II; TAF; TBN; TAFII43; TAFII-43
• NCBI Protein Information: transcription initiation factor TFIID subunit 8
• UniProt Protein Name: Transcription initiation factor TFIID subunit 8
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF8
• UniProt Synonym Gene Names: TAFII43; TBN; TAFII-43; TAFII43; hTAFII43
• UniProt Entry Name: TAF8_HUMAN

NCBI Description

This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TBN: Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. Mediates both basal and activator-dependent transcription. Plays a role in the differentiation of preadipocyte fibroblasts to adipocytes, however, does not seem to play a role in differentiation of myoblasts. Required for the integration of TAF10 in the TAF complex. May be important for survival of cells of the inner cell mass which constitute the pluripotent cell population of the early embryo. Belongs to the TAF8 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; nucleus; perinuclear region of cytoplasm; transcription factor TFIID complex

Molecular Function: protein binding; protein heterodimerization activity

Biological Process: cell differentiation; inner cell mass cell proliferation; maintenance of protein localization in nucleus; positive regulation of transcription, DNA-dependent; regulation of fat cell differentiation; snRNA transcription from RNA polymerase II promoter