Western Blot (WB)
((0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat TFB1M Polyclonal Antibody | anti-TFB1M antibody
Goat anti-TFB1M Antibody
Western Blot: Approx 38kDa band observed in lysates of cell line A431 (calculated MW of 39.5kDa according to NP_057104.2). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a dimethyltransferase that methylates the conserved stem loop of mitochondrial 12S rRNA. The encoded protein also is part of the basal mitochondrial transcription complex and is necessary for mitochondrial gene expression. The methylation and transcriptional activities of this protein are independent of one another. Variations in this gene may influence the severity of aminoglycoside-induced deafness (AID).[provided by RefSeq, Aug 2010]
Uniprot Description
TFB1M: S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Variations in TFB1M may influence the clinical expression of aminoglycoside-induced deafness caused by the A1555G mutation in the mitochondrial 12S rRNA. Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
Protein type: Methyltransferase; EC 2.1.1.-
Chromosomal Location of Human Ortholog: 6q25.1-q25.3
Cellular Component: mitochondrial matrix
Molecular Function: DNA binding; protein binding; rRNA (adenine-N6,N6-)-dimethyltransferase activity
Biological Process: mitochondrion organization and biogenesis; regulation of transcription, DNA-dependent; rRNA methylation; transcription, DNA-dependent
Research Articles on TFB1M
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Product Notes
The TFB1M tfb1m (Catalog #AAA421689) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TFB1M Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's TFB1M can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 38kDa band observed in lysates of cell line A431 (calculated MW of 39.5kDa according to NP_057104.2). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the TFB1M tfb1m for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ELKRRKSKNE EKE. It is sometimes possible for the material contained within the vial of "TFB1M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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