Goat Adenosine A1 Polyclonal Antibody | anti-ADORA1 antibody

Goat anti-Adenosine A1 receptor Antibody

Cat. Num. AAA421557

• Gene Names: ADORA1; RDC7
• Reactivity: Expected from sequence similarity: Human, Dog, Cow
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: Adenosine A1; Polyclonal Antibody; Goat anti-Adenosine A1 receptor Antibody; ADORA1; adenosine A1 receptor; RDC7; ADORA1 antibody; adenosine A1 receptor antibody; RDC7 antibody; Adenosine A1 receptor; anti-ADORA1 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: NDHFRCQPAPPIDED
• Sequence Length: 326

• Applicable Applications for anti-ADORA1 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Western Blot: Preliminary experiments gave bands at approx 30kDa and 20kDa in Human Brain (Cerebellum) lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 36.5kDa according to NP_000665.1 and NP_001041695.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
• Immunogen: Peptide with sequence NDHFRCQPAPPIDED, from the C Terminus of the protein sequence according to NP_000665.1; NP_001041695.1.
• Epitope: C Terminus
• Note: Both reported isoforms represent identical protein (NP_000665.1; NP_001041695.1)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 4501947
• NCBI GeneID: 134
• NCBI Accession #: NP_000665.1; NP_001041695.1
• NCBI GenBank Nucleotide #: NM_000674.2
• Molecular Weight: 13,348 Da
• NCBI Official Full Name: adenosine receptor A1
• NCBI Official Synonym Full Names: adenosine A1 receptor
• NCBI Official Symbol: ADORA1
• NCBI Official Synonym Symbols: RDC7
• NCBI Protein Information: adenosine receptor A1
• UniProt Protein Name: Adenosine receptor A1
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA1
• UniProt Entry Name: AA1R_HUMAN

NCBI Description

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ADORA1: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Endoplasmic reticulum; Membrane protein, multi-pass; Apoptosis; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: asymmetric synapse; axolemma; basolateral plasma membrane; cell soma; dendritic spine; endoplasmic reticulum; integral to plasma membrane; plasma membrane; postsynaptic density; postsynaptic membrane; presynaptic active zone; presynaptic membrane; terminal button

Molecular Function: adenosine receptor activity, G-protein coupled; G-protein beta/gamma-subunit binding; G-protein-coupled receptor binding; heat shock protein binding; heterotrimeric G-protein binding; phospholipase C activity; protein binding; protein heterodimerization activity; purine nucleoside binding

Biological Process: activation of MAPKK activity; adenosine receptor signaling pathway; cell-cell signaling; cognition; detection of temperature stimulus involved in sensory perception of pain; G-protein signaling, adenylate cyclase inhibiting pathway; inflammatory response; lipid catabolic process; negative regulation of acute inflammatory response; negative regulation of apoptosis; negative regulation of blood pressure; negative regulation of cell proliferation; negative regulation of circadian sleep/wake cycle, non-REM sleep; negative regulation of glutamate secretion; negative regulation of hormone secretion; negative regulation of leukocyte migration; negative regulation of lipid catabolic process; negative regulation of neurotrophin production; negative regulation of synaptic transmission, GABAergic; negative regulation of synaptic transmission, glutamatergic; negative regulation of vasodilation; nervous system development; neurological control of breathing; phagocytosis; positive regulation of blood pressure; positive regulation of epidermal growth factor receptor activity; positive regulation of nucleoside transport; positive regulation of peptide secretion; positive regulation of potassium ion transport; positive regulation of protein amino acid dephosphorylation; protein targeting to membrane; regulation of excitatory postsynaptic membrane potential; regulation of glomerular filtration; regulation of sensory perception of pain; relaxation of vascular smooth muscle; response to hypoxia; signal transduction; thermoregulation