Western Blot (WB)
((0.1ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat LIS1/PAFAH1B1 Polyclonal Antibody | anti-PAFAH1B1 antibody
Goat anti-LIS1/PAFAH1B1 Antibody
Western Blot: Approx 40kDa band observed in Human Lung and Ovary and in Rat Ovary lysates (calculated MW of 46.6kDa according to Human NP_000421.1 and to Rat NP_113951.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This locus was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. This gene encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist: one composed of multiple subunits, the other, a single subunit. In addition, a single-subunit isoform of this enzyme is found in serum. [provided by RefSeq, Apr 2009]
Uniprot Description
PAFAH1B1: Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet- activating factor (PAF) by removing the acetyl group at the SN-2 position. Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Defects in PAFAH1B1 are the cause of lissencephaly type 1 (LIS1); also known as classic lissencephaly. LIS1 is characterized by agyria or pachgyria and disorganization of the clear neuronal lamination of normal six-layered cortex. The cortex is abnormally thick and poorly organized with 4 primitive layers. LIS1 is associated with enlarged and dysmorphic ventricles and often hypoplasia of the corpus callosum. Defects in PAFAH1B1 are the cause of subcortical band heterotopia (SBH). SBH is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric ribbons of gray matter found in the central white matter between the cortex and the ventricular surface. Defects in PAFAH1B1 are a cause of Miller-Dieker lissencephaly syndrome (MDLS). MDLS is a contiguous gene deletion syndrome of chromosome 17p13.3, characterized by classical lissencephaly and distinct facial features. Additional congenital malformations can be part of the condition. Belongs to the WD repeat LIS1/nudF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell cycle regulation; Lipid Metabolism - ether lipid
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: astral microtubule; cell cortex; cell soma; centrosome; cytosol; growth cone; kinesin complex; kinetochore; leading edge; microtubule associated complex; motile primary cilium; nuclear envelope; nuclear membrane; perinuclear region of cytoplasm
Molecular Function: dynein binding; dynein intermediate chain binding; heparin binding; microtubule binding; phospholipase A2 activity; phospholipase binding; phosphoprotein binding; protein binding; protein homodimerization activity
Biological Process: acrosome formation; actin cytoskeleton organization and biogenesis; adult locomotory behavior; ameboidal cell migration; auditory receptor cell development; brain morphogenesis; cerebral cortex development; cerebral cortex neuron differentiation; corpus callosum morphogenesis; cortical microtubule organization and biogenesis; establishment of centrosome localization; establishment of mitotic spindle orientation; establishment of polarity of embryonic epithelium; G2/M transition of mitotic cell cycle; germ cell development; hippocampus development; layer formation in the cerebral cortex; learning and/or memory; lipid catabolic process; microtubule cytoskeleton organization and biogenesis; microtubule organizing center organization and biogenesis; microtubule-based process; mitosis; negative regulation of JNK cascade; neuroblast proliferation; neuromuscular process controlling balance; neuron migration; nuclear envelope disassembly; nuclear migration; platelet activating factor metabolic process; positive regulation of axon extension; positive regulation of cytokine and chemokine mediated signaling pathway; positive regulation of embryonic development; positive regulation of mitotic cell cycle; protein secretion; regulation of GTPase activity; retrograde axon cargo transport; sister chromatid cohesion; stem cell division; synaptic transmission; transmission of nerve impulse; vesicle transport along microtubule
Disease: Lissencephaly 1
Research Articles on PAFAH1B1
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Product Notes
The PAFAH1B1 pafah1b1 (Catalog #AAA421282) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-LIS1/PAFAH1B1 Antibody reacts with Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's LIS1/PAFAH1B1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 40kDa band observed in Human Lung and Ovary and in Rat Ovary lysates (calculated MW of 46.6kDa according to Human NP_000421.1 and to Rat NP_113951.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the PAFAH1B1 pafah1b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TGSVDQTVKV WECR. It is sometimes possible for the material contained within the vial of "LIS1/PAFAH1B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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