Goat EYA4 Polyclonal Antibody | anti-EYA4 antibody
Goat anti-EYA4 Antibody
Western Blot: Preliminary experiments gave an approx 50kDa band in human and mouse brain lysates and in pituitary mouse cell line NIH 3T3 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 69.5kDa, 67.1kDa and 69.4kDa according to NP_004091.2, NP_742102.1 and NP_742103.1 respectively. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant non-syndromic sensorineural 10 locus. The encoded protein is also a putative oncogene that mediates DNA repair, apoptosis, and innate immunity following DNA damage, cellular damage, and viral attack. Defects in this gene are also associated with dilated cardiomyopathy 1J. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
Uniprot Description
EYA4: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye. Defects in EYA4 are the cause of deafness autosomal dominant type 10 (DFNA10). DFNA10 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in EYA4 are the cause of cardiomyopathy dilated type 1J (CMD1J). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the HAD-like hydrolase superfamily. EYA family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48
Chromosomal Location of Human Ortholog: 6q23
Cellular Component: cytoplasm; nucleus
Molecular Function: metal ion binding; protein binding; protein tyrosine phosphatase activity
Biological Process: anatomical structure morphogenesis; chromatin modification; DNA repair; multicellular organismal development; regulation of transcription, DNA-dependent; sensory perception of sound; transcription, DNA-dependent; visual perception
Disease: Cardiomyopathy, Dilated, 1j; Deafness, Autosomal Dominant 10
Research Articles on EYA4
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Product Notes
The EYA4 eya4 (Catalog #AAA420684) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-EYA4 Antibody reacts with Expected from sequence similarity: Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EYA4 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Preliminary experiments gave an approx 50kDa band in human and mouse brain lysates and in pituitary mouse cell line NIH 3T3 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 69.5kDa, 67.1kDa and 69.4kDa according to NP_004091.2, NP_742102.1 and NP_742103.1 respectively. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the EYA4 eya4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EDSQDLNEQS VKKTC. It is sometimes possible for the material contained within the vial of "EYA4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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