Western Blot (WB)
((0.5ug/ml) staining of lysate of cell line Jurkat (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Kinesin 1/UKHC Polyclonal Antibody | anti-KIF5B antibody
Goat anti-Kinesin 1/UKHC Antibody
Gene Names
KIF5B; KNS; KINH; KNS1; UKHC; HEL-S-61
Reactivity
Tested: Human, Dog; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Kinesin 1/UKHC; Polyclonal Antibody; Goat anti-Kinesin 1/UKHC Antibody; KIF5B; UKHC; kinesin 1 (110-120kD); kinesin family member 5B; KNS; KINH; KNS1; U-KHC; kinesin heavy chain; KIF5B antibody; UKHC antibody; kinesin 1 (110-120kD) antibody; kinesin family member 5B antibody; KNS antibody; KINH antibody; KNS1 antibody; U-KHC antibody; kinesin heavy chain antibody; Kinesin 1; Kinesin 1 / UKHC; anti-KIF5B antibody
Host
Goat
Reactivity
Tested: Human, Dog; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
QPVAVRGGGGKQV
Sequence Length
963
Applicable Applications for anti-KIF5B antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration, 1-2ug/ml. Please note this data was obtained with a previous batch (different goat).
Western Blot: Approx 110kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110kDa according to NP_004512.1). Recommended concentration: 0.5-1.5ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration, 1-2ug/ml. Please note this data was obtained with a previous batch (different goat).
Western Blot: Approx 110kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110kDa according to NP_004512.1). Recommended concentration: 0.5-1.5ug/ml.
Immunogen
Peptide with sequence C-QPVAVRGGGGKQV, from the C Terminus of the protein sequence according to NP_004512.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5ug/ml) staining of lysate of cell line Jurkat (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of lysate of cell line Jurkat (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((1ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Please note this data was obtained with a previous batch.)
Immunohistochemistry (IHC)
((1ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Please note this data was obtained with a previous batch.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Please note this data was obtained with a previous batch.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Please note this data was obtained with a previous batch.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,685 Da
NCBI Official Full Name
kinesin-1 heavy chain
NCBI Official Synonym Full Names
kinesin family member 5B
NCBI Official Symbol
KIF5B
NCBI Official Synonym Symbols
KNS; KINH; KNS1; UKHC; HEL-S-61
NCBI Protein Information
kinesin-1 heavy chain
UniProt Protein Name
Kinesin-1 heavy chain
Protein Family
UniProt Gene Name
KIF5B
UniProt Synonym Gene Names
KNS; KNS1; UKHC
UniProt Entry Name
KINH_HUMAN
Uniprot Description
KIF5B: Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Belongs to the kinesin-like protein family. Kinesin subfamily.
Protein type: Motor; Microtubule-binding
Chromosomal Location of Human Ortholog: 10p11.22
Cellular Component: ciliary rootlet; cytoplasm; endocytic vesicle; kinesin complex; membrane; microtubule; microtubule organizing center; neuron projection; perinuclear region of cytoplasm; vesicle
Molecular Function: ATP binding; microtubule binding; microtubule motor activity; plus-end-directed microtubule motor activity; protein binding
Biological Process: axon guidance; cytoplasm organization and biogenesis; cytoskeleton-dependent intracellular transport; metabolic process; microtubule-based movement; positive regulation of potassium ion transport; positive regulation of synaptic transmission, GABAergic; regulation of membrane potential; vesicle transport along microtubule
Research Articles on KIF5B
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Product Notes
The KIF5B kif5b (Catalog #AAA420641) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Kinesin 1/UKHC Antibody reacts with Tested: Human, Dog; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Kinesin 1/UKHC can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Kidney, shows perinuclear labelling of some glomerular cells and cytoplasmic staining of renal tubules. Recommended concentration, 1-2ug/ml. Please note this data was obtained with a previous batch (different goat). Western Blot: Approx 110kDa band observed in lysates of the cell lines Jurkat and HeLa (calculated MW of 110kDa according to NP_004512.1). Recommended concentration: 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the KIF5B kif5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QPVAVRGGGG KQV. It is sometimes possible for the material contained within the vial of "Kinesin 1/UKHC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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