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Western Blot (WB) (Staining (2ug/ml) of Human Testis lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Goat ARL4A Polyclonal Antibody | anti-ARL4A antibody

Goat anti-ARL4A Antibody

Gene Names
ARL4A; ARL4
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ARL4A; Polyclonal Antibody; Goat anti-ARL4A Antibody; ARL4; ADP-ribosylation factor-like 4; ADP-ribosylation factor-like 4A; OTTHUMP00000155200; OTTHUMP00000201575; ARL4A antibody; ARL4 antibody; ADP-ribosylation factor-like 4 antibody; ADP-ribosylation factor-like 4A antibody; OTTHUMP00000155200 antibody; OTTHUMP00000201575 antibody; anti-ARL4A antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
GNGLSDQTSILSN
Sequence Length
200
Applicable Applications for anti-ARL4A antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Approx 23kDa band seen in Human Testis lysate. Recommended for use at 1-3ug/ml.
Immunogen
Peptide with sequence GNGLSDQTSILSN-C, from the N Terminus of the protein sequence according to NP_001032241.1; NP_005729.1; P_997625.1.
Epitope
N Terminus
Note
This is also expected to react with a hypothetical human protein, XP_166703, and a hypothetical mouse protein, AAH03725 - both very similar to ARL4.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

(Staining (2ug/ml) of Human Testis lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Western Blot (WB) (Staining (2ug/ml) of Human Testis lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,615 Da
NCBI Official Full Name
ADP-ribosylation factor-like protein 4A
NCBI Official Synonym Full Names
ADP ribosylation factor like GTPase 4A
NCBI Official Symbol
ARL4A
NCBI Official Synonym Symbols
ARL4
NCBI Protein Information
ADP-ribosylation factor-like protein 4A
UniProt Protein Name
ADP-ribosylation factor-like protein 4A
UniProt Gene Name
ARL4A
UniProt Synonym Gene Names
ARL4
UniProt Entry Name
ARL4A_HUMAN

NCBI Description

ADP-ribosylation factor-like 4A is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL4A is similar to ARL4C and ARL4D and each has a nuclear localization signal and an unusually high guaninine nucleotide exchange rate. ARL4A is located in both the nuclear and extranuclear cell compartments. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ARL4A: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form. Belongs to the small GTPase superfamily. Arf family.

Protein type: G protein, monomeric, ARF; G protein, monomeric; Nucleolus

Chromosomal Location of Human Ortholog: 7p21.3

Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; plasma membrane

Molecular Function: GTP binding; protein binding

Biological Process: brown fat cell differentiation; protein transport; small GTPase mediated signal transduction

Research Articles on ARL4A

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Product Notes

The ARL4A arl4a (Catalog #AAA420606) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ARL4A Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's ARL4A can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 23kDa band seen in Human Testis lysate. Recommended for use at 1-3ug/ml. Researchers should empirically determine the suitability of the ARL4A arl4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GNGLSDQTSI LSN. It is sometimes possible for the material contained within the vial of "ARL4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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