Western Blot (WB)
(Staining (3ug/ml) of NCI-H460 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat ELF3/ERT/ ESX Polyclonal Antibody | anti-ELF3 antibody
Goat anti-ELF3/ERT/ ESX Antibody
Western Blot: Approx 48 kDa band observed in NCI-H460 lysate (calculated MW of 41.5kDa according to NP_004424.2). In transfected HEK293 transiently expressing ELF3 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 2-4ug/ml.
Western Blot (WB)
(Staining (3ug/ml) of NCI-H460 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(Staining (3ug/ml) of NCI-H460 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing ELF3 (RC200631) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing ELF3 (RC200631) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
Uniprot Description
ELF3: Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Motility/polarity/chemotaxis; Transcription factor
Chromosomal Location of Human Ortholog: 1q32.2
Cellular Component: cytoplasm; nucleus
Molecular Function: protein binding; transcription coactivator activity; transcription factor activity
Biological Process: blastocyst development; epidermis development; epithelial cell differentiation; extracellular matrix organization and biogenesis; inflammatory response; mammary gland involution; multicellular organismal development; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Research Articles on ELF3
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Product Notes
The ELF3 elf3 (Catalog #AAA420518) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ELF3/ERT/ ESX Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ELF3/ERT/ ESX can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 48 kDa band observed in NCI-H460 lysate (calculated MW of 41.5kDa according to NP_004424.2). In transfected HEK293 transiently expressing ELF3 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 2-4ug/ml. Researchers should empirically determine the suitability of the ELF3 elf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SGWKEEEVLQ SRN. It is sometimes possible for the material contained within the vial of "ELF3/ERT/ ESX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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