Western Blot (WB)
((0.2ug/ml) staining of Human (A), Mouse (B) and Rat (C) Kidney lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Goat CRP2/CSRP2 Polyclonal Antibody | anti-CSRP2 antibody
Goat anti-CRP2/CSRP2 Antibody
Immunohistochemistry: In paraffin embedded Human Lung shows staining of the endothelial cells of a blood vessel. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21. 0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem.2000 Feb 25;275(8): 5504-11; PMID: 10681529). In transfected HEK293 transiently expressing CRP2 a band of approx.26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.2ug/ml) staining of Human (A), Mouse (B) and Rat (C) Kidney lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
((0.2ug/ml) staining of Human (A), Mouse (B) and Rat (C) Kidney lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence)
Western Blot (WB)
(HEK293 overexpressing CRP2 (RC201565) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing CRP2 (RC201565) and probed (mock transfection in first lane), tested by Origene.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((3.8ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
CSRP2 is a member of the CSRP family of genes, encoding a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. CRP2 contains two copies of the cysteine-rich amino acid sequence motif (LIM) with putative zinc-binding activity, and may be involved in regulating ordered cell growth. Other genes in the family include CSRP1 and CSRP3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
CSRP2: a LIM protein that is differentially regulated and preferentially expressed in aortic smooth muscle cells. Drastically down-regulated in response to PDGF-BB or cell injury, that promote smooth muscle cell proliferation and dedifferentiation. Seems to play a role in the development of the embryonic vascular system.
Protein type: Cell development/differentiation
Chromosomal Location of Human Ortholog: 12q21.1
Cellular Component: focal adhesion; nucleus
Molecular Function: protein binding; zinc ion binding
Biological Process: cell differentiation; multicellular organismal development; myoblast differentiation
Research Articles on CSRP2
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Product Notes
The CSRP2 csrp2 (Catalog #AAA420443) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-CRP2/CSRP2 Antibody reacts with Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Horse and may cross-react with other species as described in the data sheet. AAA Biotech's CRP2/CSRP2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Lung shows staining of the endothelial cells of a blood vessel. Recommended concentration, 3-6ug/ml. Western Blot: Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21. 0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem.2000 Feb 25;275(8): 5504-11; PMID: 10681529). In transfected HEK293 transiently expressing CRP2 a band of approx.26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the CSRP2 csrp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ERLGIKPESV QP. It is sometimes possible for the material contained within the vial of "CRP2/CSRP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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