Goat Robo3 Polyclonal Antibody | anti-Robo3 antibody
Goat anti-Robo3 (mouse) Antibody
Gene Names
Robo3; Rig1; Rbig1; Rig-1; Robo3a; Robo3b
Reactivity
Expected from sequence similarity: Mouse, Rat
Applications
ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Robo3; Polyclonal Antibody; Goat anti-Robo3 (mouse) Antibody; roundabout homolog 3 (Drosophila); Rbig1; RIG1; Rig-1; Robo3a; Robo3b; retinoblastoma inhibiting gene 1; Robo3 antibody; roundabout homolog 3 (Drosophila) antibody; Rbig1 antibody; RIG1 antibody; Rig-1 antibody; Robo3a antibody; Robo3b antibody; retinoblastoma inhibiting gene 1 antibody; Robo3 (mouse); anti-Robo3 antibody
Host
Goat
Reactivity
Expected from sequence similarity: Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RRQKQRKELSHYT
Sequence Length
1,344
Applicable Applications for anti-Robo3 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Preliminary experiments gave an approx 75-80kDa band in Mouse Brain and Rat Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 143kDa according to NP_035378.1and AAD11628.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide.
Western Blot: Preliminary experiments gave an approx 75-80kDa band in Mouse Brain and Rat Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 143kDa according to NP_035378.1and AAD11628.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide.
Immunogen
Peptide with sequence C-RRQKQRKELSHYT, from the internal region of the protein sequence according to NP_035378.1; AAD11628.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
143,440 Da
NCBI Official Synonym Full Names
roundabout guidance receptor 3
NCBI Official Symbol
Robo3
NCBI Official Synonym Symbols
Rig1; Rbig1; Rig-1; Robo3a; Robo3b
NCBI Protein Information
roundabout homolog 3
UniProt Protein Name
Roundabout homolog 3
Protein Family
UniProt Gene Name
Robo3
UniProt Synonym Gene Names
Rbig1; Rig-1
UniProt Entry Name
ROBO3_MOUSE
Uniprot Description
ROBO3: Thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal chord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. Required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. Belongs to the immunoglobulin superfamily. ROBO family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: axon; integral to membrane; membrane
Molecular Function: protein binding
Biological Process: axon guidance; axon midline choice point recognition; cell differentiation; chemotaxis; multicellular organismal development; nervous system development; neuron migration
Research Articles on Robo3
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Product Notes
The Robo3 robo3 (Catalog #AAA420435) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Robo3 (mouse) Antibody reacts with Expected from sequence similarity: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Robo3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Preliminary experiments gave an approx 75-80kDa band in Mouse Brain and Rat Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 143kDa according to NP_035378.1and AAD11628.1. The 75-80kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the Robo3 robo3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RRQKQRKELS HYT. It is sometimes possible for the material contained within the vial of "Robo3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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