Goat CSNK1E Polyclonal Antibody | anti-CSNK1E antibody

Goat anti-CSNK1E Antibody

Cat. Num. AAA420422

• Gene Names: CSNK1E; HCKIE; CKIepsilon
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CSNK1E; Polyclonal Antibody; Goat anti-CSNK1E Antibody; casein kinase 1; epsilon; RP1-5O6.1; HCKIE; MGC10398; CKI-epsilon; OTTHUMP00000063258; OTTHUMP00000063263; casein kinase 1 epsilon; casein kinase I epsilon; CSNK1E antibody; epsilon antibody; RP1-5O6.1 antibody; HCKIE antibody; MGC10398 antibody; CKI-epsilon antibody; OTTHUMP00000063258 antibody; OTTHUMP00000063263 antibody; casein kinase 1 epsilon antibody; casein kinase I epsilon antibody; anti-CSNK1E antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: PASQTSVPFDHLGK
• Sequence Length: 416

• Applicable Applications for anti-CSNK1E antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: In transfected HEK293 transiently expressing CSNK1E a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
• Immunogen: Peptide with sequence C-PASQTSVPFDHLGK, from the C Terminus of the protein sequence according to NP_001885.1; NP_689407.1.
• Epitope: C Terminus
• Note: Both reported variants represent identical protein (NP_001885.1 and NP_689407.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

(HEK293 overexpressing CSNK1E (RC202436) and probed (mock transfection in first lane), tested by Origene.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503093
• NCBI GeneID: 1454
• NCBI Accession #: NP_001885.1; NP_689407.1
• NCBI GenBank Nucleotide #: NM_001894.4
• Molecular Weight: 47,315 Da
• NCBI Official Full Name: casein kinase I isoform epsilon
• NCBI Official Synonym Full Names: casein kinase 1 epsilon
• NCBI Official Symbol: CSNK1E
• NCBI Official Synonym Symbols: HCKIE; CKIepsilon
• NCBI Protein Information: casein kinase I isoform epsilon
• UniProt Protein Name: Casein kinase I isoform epsilon
• Protein Family: Casein kinase
• UniProt Gene Name: CSNK1E
• UniProt Synonym Gene Names: CKI-epsilon; CKIe
• UniProt Entry Name: KC1E_HUMAN

NCBI Description

The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014]

Uniprot Description

CK1E: an ubiquitous protein kinase of the CK1 family. Together with Dvl-1 and Frat-1 activate the Wnt signaling pathway. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. May play a role in cell cycle progression. Two splice variant isoforms have been described. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, BMAL1 or BMAL2, CK1-D and/or CK1-E, TIMELESS and the PER proteins. Interacts directly with PER1 and PER2 which may lead to their degradation. Interacts with SOCS3. Mutations in hamster and Drosophila orthologs have circadian rhythm phenotypes, and the circadian gene period (per) is a substrate in both human and fly. A coding SNP variant in human, which increases CK1 activity, is negatively associated with circadian disorder. LOF mutations and LOH seen in mammary ductal carcinoma.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CK1; Kinase, protein; EC 2.7.11.1; CK1 group; CK1 family

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus; ribonucleoprotein complex

Molecular Function: ATP binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: circadian regulation of gene expression; DNA repair; endocytosis; G2/M transition of mitotic cell cycle; negative regulation of protein binding; negative regulation of Wnt receptor signaling pathway; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of cell shape; regulation of circadian rhythm; rRNA processing; signal transduction; Wnt receptor signaling pathway